A CRISPR Activation Screen Identifies a Pan-avian Influenza Virus Inhibitory Host Factor.

Published

Journal Article

Influenza A virus (IAV) is a pathogen that poses significant risks to human health. It is therefore critical to develop strategies to prevent influenza disease. Many loss-of-function screens have been performed to identify the host proteins required for viral infection. However, there has been no systematic screen to identify the host factors that, when overexpressed, are sufficient to prevent infection. In this study, we used CRISPR/dCas9 activation technology to perform a genome-wide overexpression screen to identify IAV restriction factors. The major hit from our screen, B4GALNT2, showed inhibitory activity against influenza viruses with an α2,3-linked sialic acid receptor preference. B4GALNT2 overexpression prevented the infection of every avian influenza virus strain tested, including the H5, H9, and H7 subtypes, which have previously caused disease in humans. Thus, we have used CRISPR/dCas9 activation technology to identify a factor that can abolish infection by avian influenza viruses.

Full Text

Duke Authors

Cited Authors

  • Heaton, BE; Kennedy, EM; Dumm, RE; Harding, AT; Sacco, MT; Sachs, D; Heaton, NS

Published Date

  • August 2017

Published In

Volume / Issue

  • 20 / 7

Start / End Page

  • 1503 - 1512

PubMed ID

  • 28813663

Pubmed Central ID

  • 28813663

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

International Standard Serial Number (ISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2017.07.060

Language

  • eng