Embedded 3D Photopatterning of Hydrogels with Diverse and Complex Architectures for Tissue Engineering and Disease Models.

Journal Article (Journal Article)

Techniques that can create three-dimensional (3D) structures to provide architectural support for cells have a significant impact in generating complex and hierarchically organized tissues/organs. In recent times, a number of technologies, including photopatterning, have been developed to create such intricate 3D structures. In this study, we describe an easy-to-implement photopatterning approach, involving a conventional fluorescent microscope and a simple photomask, to encapsulate cells within spatially defined 3D structures. We have demonstrated the ease and the versatility of this approach by creating simple to complex as well as multilayered structures. We have extended this photopatterning approach to incorporate and spatially organize multiple cell types, thereby establishing coculture systems. Such cost-effective and easy-to-use approaches can greatly advance tissue engineering strategies.

Full Text

Duke Authors

Cited Authors

  • Davey, SK; Aung, A; Agrawal, G; Lim, HL; Kar, M; Varghese, S

Published Date

  • November 2015

Published In

Volume / Issue

  • 21 / 11

Start / End Page

  • 1188 - 1196

PubMed ID

  • 26154197

Pubmed Central ID

  • PMC4638205

Electronic International Standard Serial Number (EISSN)

  • 1937-3392

Digital Object Identifier (DOI)

  • 10.1089/ten.TEC.2015.0179


  • eng

Conference Location

  • United States