Cardiomyocyte Maturation Requires TLR3 Activated Nuclear Factor Kappa B.

Published

Journal Article

The process by which committed precursors mature into cardiomyocytes is poorly understood. We found that TLR3 inhibition blocked cardiomyocyte maturation; precursor cells committed to the cardiomyocyte lineage failed to express maturation genes and sarcomeres did not develop. Using various approaches, we found that the effects of TLR3 upon cardiomyocyte maturation were dependent upon the RelA subunit of nuclear factor kappa B (NFκB). Importantly, under conditions that promote the development of mature cardiomyocytes NFκB became significantly enriched at the promoters of cardiomyocyte maturation genes. Furthermore, activation of the TLR3-NFκB pathway enhanced cardiomyocyte maturation. This study, therefore, demonstrates that the TLR3-NFκB pathway is necessary for the maturation of committed precursors into mature cardiomyocytes. Stem Cells 2018;36:1198-1209.

Full Text

Duke Authors

Cited Authors

  • Hodgkinson, CP; Pratt, RE; Kirste, I; Dal-Pra, S; Cooke, JP; Dzau, VJ

Published Date

  • August 2018

Published In

Volume / Issue

  • 36 / 8

Start / End Page

  • 1198 - 1209

PubMed ID

  • 29676038

Pubmed Central ID

  • 29676038

Electronic International Standard Serial Number (EISSN)

  • 1549-4918

Digital Object Identifier (DOI)

  • 10.1002/stem.2833

Language

  • eng

Conference Location

  • United States