Self-Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture.

Published

Journal Article

Progress in prostate cancer research is presently limited by a shortage of reliable in vitro model systems. The authors describe a novel self-assembling peptide, bQ13, which forms nanofibers and gels useful for the 3D culture of prostate cancer spheroids, with improved cytocompatibility compared to related fibrillizing peptides. The mechanical properties of bQ13 gels can be controlled by adjusting peptide concentration, with storage moduli ranging between 1 and 10 kPa. bQ13's ability to remain soluble at mildly basic pH considerably improved the viability of encapsulated cells compared to other self-assembling nanofiber-forming peptides. LNCaP cells formed spheroids in bQ13 gels with similar morphologies and sizes to those formed in Matrigel or RADA16-I. Moreover, prostate-specific antigen (PSA) is produced by LNCaP cells in all matrices, and PSA production is more responsive to enzalutamide treatment in bQ13 gels than in other fibrillized peptide gels. bQ13 represents an attractive platform for further tailoring within 3D cell culture systems.

Full Text

Duke Authors

Cited Authors

  • Hainline, KM; Gu, F; Handley, JF; Tian, YF; Wu, Y; de Wet, L; Vander Griend, DJ; Collier, JH

Published Date

  • January 2019

Published In

Volume / Issue

  • 19 / 1

Start / End Page

  • e1800249 -

PubMed ID

  • 30324687

Pubmed Central ID

  • 30324687

Electronic International Standard Serial Number (EISSN)

  • 1616-5195

International Standard Serial Number (ISSN)

  • 1616-5187

Digital Object Identifier (DOI)

  • 10.1002/mabi.201800249

Language

  • eng