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Self-Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture.

Publication ,  Journal Article
Hainline, KM; Gu, F; Handley, JF; Tian, YF; Wu, Y; de Wet, L; Vander Griend, DJ; Collier, JH
Published in: Macromolecular bioscience
January 2019

Progress in prostate cancer research is presently limited by a shortage of reliable in vitro model systems. The authors describe a novel self-assembling peptide, bQ13, which forms nanofibers and gels useful for the 3D culture of prostate cancer spheroids, with improved cytocompatibility compared to related fibrillizing peptides. The mechanical properties of bQ13 gels can be controlled by adjusting peptide concentration, with storage moduli ranging between 1 and 10 kPa. bQ13's ability to remain soluble at mildly basic pH considerably improved the viability of encapsulated cells compared to other self-assembling nanofiber-forming peptides. LNCaP cells formed spheroids in bQ13 gels with similar morphologies and sizes to those formed in Matrigel or RADA16-I. Moreover, prostate-specific antigen (PSA) is produced by LNCaP cells in all matrices, and PSA production is more responsive to enzalutamide treatment in bQ13 gels than in other fibrillized peptide gels. bQ13 represents an attractive platform for further tailoring within 3D cell culture systems.

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Published In

Macromolecular bioscience

DOI

EISSN

1616-5195

ISSN

1616-5187

Publication Date

January 2019

Volume

19

Issue

1

Start / End Page

e1800249

Related Subject Headings

  • Spheroids, Cellular
  • Prostatic Neoplasms
  • Prostate-Specific Antigen
  • Polymers
  • Phenylthiohydantoin
  • Peptides
  • Nitriles
  • Nanofibers
  • Mice
  • Male
 

Citation

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Hainline, K. M., Gu, F., Handley, J. F., Tian, Y. F., Wu, Y., de Wet, L., … Collier, J. H. (2019). Self-Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture. Macromolecular Bioscience, 19(1), e1800249. https://doi.org/10.1002/mabi.201800249
Hainline, Kelly M., Fangqi Gu, Jacqueline F. Handley, Ye F. Tian, Yaoying Wu, Larischa de Wet, Donald J. Vander Griend, and Joel H. Collier. “Self-Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture.Macromolecular Bioscience 19, no. 1 (January 2019): e1800249. https://doi.org/10.1002/mabi.201800249.
Hainline KM, Gu F, Handley JF, Tian YF, Wu Y, de Wet L, et al. Self-Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture. Macromolecular bioscience. 2019 Jan;19(1):e1800249.
Hainline, Kelly M., et al. “Self-Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture.Macromolecular Bioscience, vol. 19, no. 1, Jan. 2019, p. e1800249. Epmc, doi:10.1002/mabi.201800249.
Hainline KM, Gu F, Handley JF, Tian YF, Wu Y, de Wet L, Vander Griend DJ, Collier JH. Self-Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture. Macromolecular bioscience. 2019 Jan;19(1):e1800249.
Journal cover image

Published In

Macromolecular bioscience

DOI

EISSN

1616-5195

ISSN

1616-5187

Publication Date

January 2019

Volume

19

Issue

1

Start / End Page

e1800249

Related Subject Headings

  • Spheroids, Cellular
  • Prostatic Neoplasms
  • Prostate-Specific Antigen
  • Polymers
  • Phenylthiohydantoin
  • Peptides
  • Nitriles
  • Nanofibers
  • Mice
  • Male