Ultrastructural Analysis of Vesicular Transport in Electrotransfection.

Published

Journal Article

Emerging evidence from various studies indicates that plasmid DNA (pDNA) is internalized by cells through an endocytosis-like process when it is used for electrotransfection. To provide morphological evidence of the process, we investigated ultrastructures in cells that were associated with the electrotransfected pDNA, using immunoelectron microscopy. The results demonstrate that four endocytic pathways are involved in the uptake of the pDNA, including caveolae- and clathrin-mediated endocytosis, macropinocytosis, and the clathrin-independent carrier/glycosylphosphatidylinositol-anchored protein-enriched early endosomal compartment (CLIC/GEEC) pathway. Among them, macropinocytosis is the most common pathway utilized by cells having various pDNA uptake capacities, and the CLIC/GEEC pathway is observed primarily in human umbilical vein endothelial cells. Quantitatively, the endocytic pathways are more active in easy-to-transfect cells than in hard-to-transfect ones. Taken together, our data provide ultrastructural evidence showing that endocytosis plays an important role in cellular uptake and intracellular transport of electrotransfected pDNA.

Full Text

Duke Authors

Cited Authors

  • Wang, L; Miller, SE; Yuan, F

Published Date

  • October 2018

Published In

Volume / Issue

  • 24 / 5

Start / End Page

  • 553 - 563

PubMed ID

  • 30334512

Pubmed Central ID

  • 30334512

Electronic International Standard Serial Number (EISSN)

  • 1435-8115

International Standard Serial Number (ISSN)

  • 1431-9276

Digital Object Identifier (DOI)

  • 10.1017/S143192761801509X

Language

  • eng