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Ultrastructural Analysis of Vesicular Transport in Electrotransfection.

Publication ,  Journal Article
Wang, L; Miller, SE; Yuan, F
Published in: Microsc Microanal
October 2018

Emerging evidence from various studies indicates that plasmid DNA (pDNA) is internalized by cells through an endocytosis-like process when it is used for electrotransfection. To provide morphological evidence of the process, we investigated ultrastructures in cells that were associated with the electrotransfected pDNA, using immunoelectron microscopy. The results demonstrate that four endocytic pathways are involved in the uptake of the pDNA, including caveolae- and clathrin-mediated endocytosis, macropinocytosis, and the clathrin-independent carrier/glycosylphosphatidylinositol-anchored protein-enriched early endosomal compartment (CLIC/GEEC) pathway. Among them, macropinocytosis is the most common pathway utilized by cells having various pDNA uptake capacities, and the CLIC/GEEC pathway is observed primarily in human umbilical vein endothelial cells. Quantitatively, the endocytic pathways are more active in easy-to-transfect cells than in hard-to-transfect ones. Taken together, our data provide ultrastructural evidence showing that endocytosis plays an important role in cellular uptake and intracellular transport of electrotransfected pDNA.

Duke Scholars

Published In

Microsc Microanal

DOI

EISSN

1435-8115

Publication Date

October 2018

Volume

24

Issue

5

Start / End Page

553 / 563

Location

England

Related Subject Headings

  • Veins
  • Transport Vesicles
  • Transfection
  • Tissue Embedding
  • Plasmids
  • Pinocytosis
  • Microscopy, Immunoelectron
  • Microscopy
  • Humans
  • Gene Transfer Techniques
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, L., Miller, S. E., & Yuan, F. (2018). Ultrastructural Analysis of Vesicular Transport in Electrotransfection. Microsc Microanal, 24(5), 553–563. https://doi.org/10.1017/S143192761801509X
Wang, Liangli, Sara E. Miller, and Fan Yuan. “Ultrastructural Analysis of Vesicular Transport in Electrotransfection.Microsc Microanal 24, no. 5 (October 2018): 553–63. https://doi.org/10.1017/S143192761801509X.
Wang L, Miller SE, Yuan F. Ultrastructural Analysis of Vesicular Transport in Electrotransfection. Microsc Microanal. 2018 Oct;24(5):553–63.
Wang, Liangli, et al. “Ultrastructural Analysis of Vesicular Transport in Electrotransfection.Microsc Microanal, vol. 24, no. 5, Oct. 2018, pp. 553–63. Pubmed, doi:10.1017/S143192761801509X.
Wang L, Miller SE, Yuan F. Ultrastructural Analysis of Vesicular Transport in Electrotransfection. Microsc Microanal. 2018 Oct;24(5):553–563.
Journal cover image

Published In

Microsc Microanal

DOI

EISSN

1435-8115

Publication Date

October 2018

Volume

24

Issue

5

Start / End Page

553 / 563

Location

England

Related Subject Headings

  • Veins
  • Transport Vesicles
  • Transfection
  • Tissue Embedding
  • Plasmids
  • Pinocytosis
  • Microscopy, Immunoelectron
  • Microscopy
  • Humans
  • Gene Transfer Techniques