Ultrastructural Analysis of Vesicular Transport in Electrotransfection.
Journal Article (Journal Article)
Emerging evidence from various studies indicates that plasmid DNA (pDNA) is internalized by cells through an endocytosis-like process when it is used for electrotransfection. To provide morphological evidence of the process, we investigated ultrastructures in cells that were associated with the electrotransfected pDNA, using immunoelectron microscopy. The results demonstrate that four endocytic pathways are involved in the uptake of the pDNA, including caveolae- and clathrin-mediated endocytosis, macropinocytosis, and the clathrin-independent carrier/glycosylphosphatidylinositol-anchored protein-enriched early endosomal compartment (CLIC/GEEC) pathway. Among them, macropinocytosis is the most common pathway utilized by cells having various pDNA uptake capacities, and the CLIC/GEEC pathway is observed primarily in human umbilical vein endothelial cells. Quantitatively, the endocytic pathways are more active in easy-to-transfect cells than in hard-to-transfect ones. Taken together, our data provide ultrastructural evidence showing that endocytosis plays an important role in cellular uptake and intracellular transport of electrotransfected pDNA.
Full Text
Duke Authors
Cited Authors
- Wang, L; Miller, SE; Yuan, F
Published Date
- October 2018
Published In
Volume / Issue
- 24 / 5
Start / End Page
- 553 - 563
PubMed ID
- 30334512
Pubmed Central ID
- 30334512
Electronic International Standard Serial Number (EISSN)
- 1435-8115
Digital Object Identifier (DOI)
- 10.1017/S143192761801509X
Language
- eng
Conference Location
- United States