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Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes.

Publication ,  Journal Article
Zubcevic, L; Hsu, AL; Borgnia, MJ; Lee, S-Y
Published in: Elife
May 15, 2019

The Transient Receptor Potential Vanilloid 2 (TRPV2) channel is a member of the temperature-sensing thermoTRPV family. Recent advances in cryo-electronmicroscopy (cryo-EM) and X-ray crystallography have provided many important insights into the gating mechanisms of thermoTRPV channels. Interestingly, crystallographic studies of ligand-dependent TRPV2 gating have shown that the TRPV2 channel adopts two-fold symmetric arrangements during the gating cycle. However, it was unclear if crystal packing forces played a role in stabilizing the two-fold symmetric arrangement of the channel. Here, we employ cryo-EM to elucidate the structure of full-length rabbit TRPV2 in complex with the agonist resiniferatoxin (RTx) in nanodiscs and amphipol. We show that RTx induces two-fold symmetric conformations of TRPV2 in both environments. However, the two-fold symmetry is more pronounced in the native-like lipid environment of the nanodiscs. Our data offers insights into a gating pathway in TRPV2 involving symmetry transitions.

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Published In

Elife

DOI

EISSN

2050-084X

Publication Date

May 15, 2019

Volume

8

Location

England

Related Subject Headings

  • TRPV Cation Channels
  • Rabbits
  • Protein Conformation
  • Protein Binding
  • Membranes
  • Diterpenes
  • Cryoelectron Microscopy
  • Animals
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
 

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Zubcevic, L., Hsu, A. L., Borgnia, M. J., & Lee, S.-Y. (2019). Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes. Elife, 8. https://doi.org/10.7554/eLife.45779
Zubcevic, Lejla, Allen L. Hsu, Mario J. Borgnia, and Seok-Yong Lee. “Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes.Elife 8 (May 15, 2019). https://doi.org/10.7554/eLife.45779.
Zubcevic L, Hsu AL, Borgnia MJ, Lee S-Y. Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes. Elife. 2019 May 15;8.
Zubcevic, Lejla, et al. “Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes.Elife, vol. 8, May 2019. Pubmed, doi:10.7554/eLife.45779.
Zubcevic L, Hsu AL, Borgnia MJ, Lee S-Y. Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes. Elife. 2019 May 15;8.

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

May 15, 2019

Volume

8

Location

England

Related Subject Headings

  • TRPV Cation Channels
  • Rabbits
  • Protein Conformation
  • Protein Binding
  • Membranes
  • Diterpenes
  • Cryoelectron Microscopy
  • Animals
  • 42 Health sciences
  • 32 Biomedical and clinical sciences