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Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice.

Publication ,  Journal Article
Lee, C-L; Daniel, AR; Holbrook, M; Brownstein, J; Silva Campos, LD; Hasapis, S; Ma, Y; Borst, LB; Badea, CT; Kirsch, DG
Published in: Radiat Res
September 2019

Exposure of the gastrointestinal (GI) tract to ionizing radiation can cause acute and delayed injury. However, critical cellular targets that regulate the development of radiation-induced GI injury remain incompletely understood. Here, we investigated the role of vascular endothelial cells in controlling acute and delayed GI injury after total-abdominal irradiation (TAI). To address this, we used genetically engineered mice in which endothelial cells are sensitized to radiation due to the deletion of the tumor suppressor p53. Remarkably, we found that VE-cadherin-Cre; p53FL/FL mice, in which both alleles of p53 are deleted in endothelial cells, were not sensitized to the acute GI radiation syndrome, but these mice were highly susceptible to delayed radiation enteropathy. Histological examination indicated that VE-cadherin-Cre; p53FL/FL mice that developed delayed radiation enteropathy had severe vascular injury in the small intestine, which was manifested by hemorrhage, loss of microvessels and tissue hypoxia. In addition, using dual-energy CT imaging, we showed that VE-cadherin-Cre; p53FL/FL mice had a significant increase in vascular permeability of the small intestine in vivo 28 days after TAI. Together, these findings demonstrate that while sensitization of endothelial cells to radiation does not exacerbate the acute GI radiation syndrome, it is sufficient to promote the development of late radiation enteropathy.

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Published In

Radiat Res

DOI

EISSN

1938-5404

Publication Date

September 2019

Volume

192

Issue

3

Start / End Page

258 / 266

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Time Factors
  • Radiation Tolerance
  • Oncology & Carcinogenesis
  • Mice
  • Intestines
  • Gene Deletion
  • Endothelial Cells
  • Cell Hypoxia
  • Capillary Permeability
 

Citation

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Lee, C.-L., Daniel, A. R., Holbrook, M., Brownstein, J., Silva Campos, L. D., Hasapis, S., … Kirsch, D. G. (2019). Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice. Radiat Res, 192(3), 258–266. https://doi.org/10.1667/RR15371.1
Lee, Chang-Lung, Andrea R. Daniel, Matt Holbrook, Jeremy Brownstein, Lorraine Da Silva Campos, Stephanie Hasapis, Yan Ma, Luke B. Borst, Cristian T. Badea, and David G. Kirsch. “Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice.Radiat Res 192, no. 3 (September 2019): 258–66. https://doi.org/10.1667/RR15371.1.
Lee C-L, Daniel AR, Holbrook M, Brownstein J, Silva Campos LD, Hasapis S, et al. Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice. Radiat Res. 2019 Sep;192(3):258–66.
Lee, Chang-Lung, et al. “Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice.Radiat Res, vol. 192, no. 3, Sept. 2019, pp. 258–66. Pubmed, doi:10.1667/RR15371.1.
Lee C-L, Daniel AR, Holbrook M, Brownstein J, Silva Campos LD, Hasapis S, Ma Y, Borst LB, Badea CT, Kirsch DG. Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice. Radiat Res. 2019 Sep;192(3):258–266.

Published In

Radiat Res

DOI

EISSN

1938-5404

Publication Date

September 2019

Volume

192

Issue

3

Start / End Page

258 / 266

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Time Factors
  • Radiation Tolerance
  • Oncology & Carcinogenesis
  • Mice
  • Intestines
  • Gene Deletion
  • Endothelial Cells
  • Cell Hypoxia
  • Capillary Permeability