Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice.

Journal Article (Journal Article)

Exposure of the gastrointestinal (GI) tract to ionizing radiation can cause acute and delayed injury. However, critical cellular targets that regulate the development of radiation-induced GI injury remain incompletely understood. Here, we investigated the role of vascular endothelial cells in controlling acute and delayed GI injury after total-abdominal irradiation (TAI). To address this, we used genetically engineered mice in which endothelial cells are sensitized to radiation due to the deletion of the tumor suppressor p53. Remarkably, we found that VE-cadherin-Cre; p53FL/FL mice, in which both alleles of p53 are deleted in endothelial cells, were not sensitized to the acute GI radiation syndrome, but these mice were highly susceptible to delayed radiation enteropathy. Histological examination indicated that VE-cadherin-Cre; p53FL/FL mice that developed delayed radiation enteropathy had severe vascular injury in the small intestine, which was manifested by hemorrhage, loss of microvessels and tissue hypoxia. In addition, using dual-energy CT imaging, we showed that VE-cadherin-Cre; p53FL/FL mice had a significant increase in vascular permeability of the small intestine in vivo 28 days after TAI. Together, these findings demonstrate that while sensitization of endothelial cells to radiation does not exacerbate the acute GI radiation syndrome, it is sufficient to promote the development of late radiation enteropathy.

Full Text

Duke Authors

Cited Authors

  • Lee, C-L; Daniel, AR; Holbrook, M; Brownstein, J; Silva Campos, LD; Hasapis, S; Ma, Y; Borst, LB; Badea, CT; Kirsch, DG

Published Date

  • September 2019

Published In

Volume / Issue

  • 192 / 3

Start / End Page

  • 258 - 266

PubMed ID

  • 31265788

Pubmed Central ID

  • PMC6776243

Electronic International Standard Serial Number (EISSN)

  • 1938-5404

Digital Object Identifier (DOI)

  • 10.1667/RR15371.1


  • eng

Conference Location

  • United States