Chang Lung Lee | Scholars@Duke

Chang Lung Lee
Assistant Professor in Radiation Oncology

The overall goal of the Lee lab’s research program is to improve the survivorship of cancer patients by minimizing acute and long-term side effects of radiation therapy. We are also investigating novel medical countermeasures against acute radiation syndrome in the scenarios of nuclear terrorism.

Current Research Interests

Utilizing sophisticated mouse models, we aim to study 1) critical signaling pathways that regulate the regeneration of normal tissues following radiation injury; 2) cell-autonomous and non-cell-autonomous mechanisms underlying the development of radiation-induced cancer.

Ongoing projects:
1. Radiation-induced hematologic malignancies and clonal hematopoiesis
2. Radiation-induced acute and chronic intestinal injury
3. Radiation response of rectal cancer (collaboration with Jatin Roper, MD)

Current Appointments & Affiliations

Assistant Professor in Radiation Oncology, Radiation Oncology, Clinical Science Departments 2021
Assistant Professor in Pathology, Pathology, Clinical Science Departments 2020
Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 2018

Contact Information

B220 LSRC Bldg, 308 Research Dr, Box 3813 Med Ctr, Durham, NC 27708
Box 3813 Med Ctr, Durham, NC 27708
chang-lung.lee@duke.edu (919) 684-8145
Lee lab webpage

Background
Education, Training, & Certifications
- Postdoctoral Associate, Radiation Oncology/Kirsch Laboratory, Duke University School of Medicine 2012 - 2017
- Ph.D., Duke University 2012
Previous Appointments & Affiliations
- Assistant Professor in Radiation Oncology, Radiation Oncology, Clinical Science Departments 2018 - 2020
- Assistant Professor - Track 5 in Pathology, Pathology, Clinical Science Departments 2018 - 2020
Recognition
Awards & Honors
Expertise
Subject Headings
Research
Selected Grants
- Cabinet Radiation Therapy System for Small Animals awarded by North Carolina Biotechnology Center 2022 - 2023
- Inhibition of CaMKK2 sensitizes rectal cancers to radiation therapy awarded by National Institutes of Health 2021 - 2022
- Minimizing the risk of therapy-related myeloid neoplasms by inhibiting genotoxic stress-induced expansion of leukemia-initiating cells awarded by National Institutes of Health 2020 - 2022
- Mitigating the gastrointestinal acute radiation syndrome by activating Yap1 awarded by Columbia University 2021 - 2022
- Image-Guided Radiation Therapy System for Small Animals awarded by National Institutes of Health 2021 - 2022
- Dissecting mechanism(s) by which ionizing radiation promotes clonal expansion of premalignant cells in the thymus awarded by National Institutes of Health 2018 - 2020
- Mitigating the gastrointestinal acute radiation syndrome by blocking calcium/calmodulin-dependent protein kinase kinase 2 awarded by Department of Defense 2019 - 2020
- Treatment of gastrointestinal acute radiation syndrome with activation of peroxisome proliferator-activated receptor delta awarded by Columbia University 2019 - 2020
- Development of CaMKK2 inhibitor drug for acute radiation syndrome awarded by Columbia University 2018 - 2020
- Dissecting mechanism(s) by which ionizing radiation promotes clonal expansion of premalignant cells in the thymus awarded by National Institutes of Health 2016 - 2018
Fellowships, Supported Research, & Other Grants
- The effect of RAD-100 on a murine model of radiation dermatitis awarded by Rythera Therapeutics, Inc 2021 - 2022
- Determining the impact of thrombopoietin mimetic (TPOm) on the response of human prostate tumors to radiation therapy awarded by Janssen Research & Development, LLC 2019 - 2020
- Increasing the therapeutic ratio of pelvic radiotherapy for rectal cancer by blocking calcium/calmodulin-dependent protein kinase kinase 2 awarded by Duke Cancer Institute 2019 - 2020
Publications & Artistic Works
Selected Publications
- Academic Articles
  - Kuo, Hsuan-Cheng, Lixia Luo, Yan Ma, Nerissa Williams, Lorraine da Silva Campos, Laura Attardi, Chang-Lung Lee, and David Kirsch. “The p53 transactivation domain 1-dependent response to acute DNA damage in endothelial cells protects against radiation-induced cardiac injury,” December 5, 2021. https://doi.org/10.1101/2021.12.04.471191.
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  - Daniel, Andrea R., Lixia Luo, Chang-Lung Lee, and David G. Kirsch. “Investigating the Role of Inflammasome Caspases 1 and 11 in the Acute Radiation Syndrome.” Radiat Res 196, no. 6 (December 1, 2021): 686–89. https://doi.org/10.1667/RADE-21-00141.1.
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  - Lee, Chang-Lung, Ato O. Wright, Jessica W. Lee, Jeremy Brownstein, Stephanie Hasapis, Sloane Satow, Lorraine Da Silva Campos, et al. “Sensitization of Endothelial Cells to Ionizing Radiation Exacerbates Delayed Radiation Myelopathy in Mice.” Radiation Research 197, no. 3 (November 2021): 0. https://doi.org/10.1667/rade-21-00166.1.
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  - Lee, Chang-Lung, Kennedy D. Brock, Stephanie Hasapis, Dadong Zhang, Alexander B. Sibley, Xiaodi Qin, Jeremy S. Gresham, et al. “Whole-Exome Sequencing of Radiation-Induced Thymic Lymphoma in Mouse Models Identifies Notch1 Activation as a Driver of p53 Wild-Type Lymphoma.” Cancer Res 81, no. 14 (July 15, 2021): 3777–90. https://doi.org/10.1158/0008-5472.CAN-20-2823.
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  - Kam, Angel Y. F., Sadhna O. Piryani, Chang-Lung Lee, David A. Rizzieri, Neil L. Spector, Stefanie Sarantopoulos, and Phuong L. Doan. “Selective ERBB2 and BCL2 Inhibition Is Synergistic for Mitochondrial-Mediated Apoptosis in MDS and AML Cells.” Mol Cancer Res 19, no. 5 (May 2021): 886–99. https://doi.org/10.1158/1541-7786.MCR-20-0973.
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  - Lee, Chang-Lung, Jessica W. Lee, Andrea R. Daniel, Matt Holbrook, Stephanie Hasapis, Ato O. Wright, Jeremy Brownstein, et al. “Characterization of cardiovascular injury in mice following partial-heart irradiation with clinically relevant dose and fractionation.” Radiother Oncol 157 (April 2021): 155–62. https://doi.org/10.1016/j.radonc.2021.01.023.
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  - Hasapis, Stephanie, Isibel Caraballo, and Chang-Lung Lee. “Transplantation of Unirradiated Bone Marrow Cells after Total-Body Irradiation Prevents the Development of Thymic Lymphoma in Mice through Niche Competition.” Radiat Res 195, no. 3 (March 1, 2021): 301–6. https://doi.org/10.1667/RADE-20-00221.1.
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  - Sheahan, Breanna J., Ally N. Freeman, Theresa M. Keeley, Linda C. Samuelson, Jatin Roper, Stephanie Hasapis, Chang-Lung Lee, and Christopher M. Dekaney. “Epithelial Regeneration After Doxorubicin Arises Primarily From Early Progeny of Active Intestinal Stem Cells.” Cell Mol Gastroenterol Hepatol 12, no. 1 (2021): 119–40. https://doi.org/10.1016/j.jcmgh.2021.01.015.
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  - Daniel, Andrea R., Chang-Lung Lee, Patrick Oh, Lixia Luo, Yan Ma, and David G. Kirsch. “Inhibiting Glycogen Synthase Kinase-3 Mitigates the Hematopoietic Acute Radiation Syndrome in a Sex- and Strain-dependent Manner in Mice.” Health Phys 119, no. 3 (September 2020): 315–21. https://doi.org/10.1097/HP.0000000000001243.
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  - Lee, Chang-Lung, Kennedy Davis Brock, Stephanie Hasapis, Dadong Zhang, Alexander Sibley, Xiaodi Qin, Jeremy Gresham, et al. “Activation of Notch1 drives the development of radiation-induced thymic lymphoma in p53 wild-type mice,” August 21, 2020. https://doi.org/10.1101/2020.08.21.261487.
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  - Tang, Yuning J., Jianguo Huang, Hidetoshi Tsushima, Ga I. Ban, Hongyuan Zhang, Kristianne M. Oristian, Vijitha Puviindran, et al. “Tracing Tumor Evolution in Sarcoma Reveals Clonal Origin of Advanced Metastasis.” Cell Rep 28, no. 11 (September 10, 2019): 2837-2850.e5. https://doi.org/10.1016/j.celrep.2019.08.029.
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  - Lee, Chang-Lung, Andrea R. Daniel, Matt Holbrook, Jeremy Brownstein, Lorraine Da Silva Campos, Stephanie Hasapis, Yan Ma, Luke B. Borst, Cristian T. Badea, and David G. Kirsch. “Sensitization of Vascular Endothelial Cells to Ionizing Radiation Promotes the Development of Delayed Intestinal Injury in Mice.” Radiat Res 192, no. 3 (September 2019): 258–66. https://doi.org/10.1667/RR15371.1.
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  - Lee, Chang-Lung, Yvonne M. Mowery, Andrea R. Daniel, Dadong Zhang, Alexander B. Sibley, Joe R. Delaney, Amy J. Wisdom, et al. “Mutational landscape in genetically engineered, carcinogen-induced, and radiation-induced mouse sarcoma.” Jci Insight 4, no. 13 (July 11, 2019). https://doi.org/10.1172/jci.insight.128698.
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  - Clark, Darin, Matthew Holbrook, Chang-Lung Lee, and Cristian Badea. “Photon-counting cine-cardiac CT in the mouse,” June 4, 2019. https://doi.org/10.1101/660100.
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  - Torok, Jordan A., Patrick Oh, Katherine D. Castle, Michael Reinsvold, Yan Ma, Lixia Luo, Chang-Lung Lee, and David G. Kirsch. “Deletion of Atm in Tumor but not Endothelial Cells Improves Radiation Response in a Primary Mouse Model of Lung Adenocarcinoma.” Cancer Res 79, no. 4 (February 15, 2019): 773–82. https://doi.org/10.1158/0008-5472.CAN-17-3103.
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  - Clark, Darin P., Matthew Holbrook, Chang-Lung Lee, and Cristian T. Badea. “Photon-counting cine-cardiac CT in the mouse.” Plos One 14, no. 9 (2019): e0218417. https://doi.org/10.1371/journal.pone.0218417.
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  - Lee, Chang-Lung, Patrick Oh, Eric S. Xu, Yan Ma, Yongbaek Kim, Andrea R. Daniel, and David G. Kirsch. “Blocking Cyclin-Dependent Kinase 4/6 During Single Dose Versus Fractionated Radiation Therapy Leads to Opposite Effects on Acute Gastrointestinal Toxicity in Mice.” Int J Radiat Oncol Biol Phys 102, no. 5 (December 1, 2018): 1569–76. https://doi.org/10.1016/j.ijrobp.2018.07.192.
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  - Cheung, Phyllis F., Florian Neff, Christian Neander, Anna Bazarna, Konstantinos Savvatakis, Sven-Thorsten Liffers, Kristina Althoff, et al. “Notch-Induced Myeloid Reprogramming in Spontaneous Pancreatic Ductal Adenocarcinoma by Dual Genetic Targeting.” Cancer Res 78, no. 17 (September 1, 2018): 4997–5010. https://doi.org/10.1158/0008-5472.CAN-18-0052.
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  - Castle, Katherine D., Andrea R. Daniel, Everett J. Moding, Lixia Luo, Chang-Lung Lee, and David G. Kirsch. “Mice Lacking RIP3 Kinase are not Protected from Acute Radiation Syndrome.” Radiat Res 189, no. 6 (June 2018): 627–33. https://doi.org/10.1667/RR15001.1.
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  - Brownstein, Jeremy M., Amy J. Wisdom, Katherine D. Castle, Yvonne M. Mowery, Peter Guida, Chang-Lung Lee, Francesco Tommasino, et al. “Characterizing the Potency and Impact of Carbon Ion Therapy in a Primary Mouse Model of Soft Tissue Sarcoma.” Mol Cancer Ther 17, no. 4 (April 2018): 858–68. https://doi.org/10.1158/1535-7163.MCT-17-0965.
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  - Dodd, Rebecca D., Chang-Lung Lee, Tess Overton, Wesley Huang, William C. Eward, Lixia Luo, Yan Ma, et al. “NF1+/- Hematopoietic Cells Accelerate Malignant Peripheral Nerve Sheath Tumor Development without Altering Chemotherapy Response.” Cancer Res 77, no. 16 (August 15, 2017): 4486–97. https://doi.org/10.1158/0008-5472.CAN-16-2643.
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  - Moding, Everett J., Hooney D. Min, Katherine D. Castle, Moiez Ali, Loretta Woodlief, Nerissa Williams, Yan Ma, Yongbaek Kim, Chang-Lung Lee, and David G. Kirsch. “An extra copy of p53 suppresses development of spontaneous Kras-driven but not radiation-induced cancer.” Jci Insight 1, no. 10 (July 7, 2016). https://doi.org/10.1172/jci.insight.86698.
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  - Sachdeva, Mohit, Jeffrey K. Mito, Chang-Lung Lee, Minsi Zhang, Zhizhong Li, Rebecca D. Dodd, David Cason, et al. “MicroRNA-182 drives metastasis of primary sarcomas by targeting multiple genes.” J Clin Invest 126, no. 4 (April 1, 2016): 1606. https://doi.org/10.1172/JCI86573.
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  - Whitley, Melodi Javid, Diana M. Cardona, Alexander L. Lazarides, Ivan Spasojevic, Jorge M. Ferrer, Joan Cahill, Chang-Lung Lee, et al. “A mouse-human phase 1 co-clinical trial of a protease-activated fluorescent probe for imaging cancer.” Sci Transl Med 8, no. 320 (January 6, 2016): 320ra4. https://doi.org/10.1126/scitranslmed.aad0293.
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  - Clark, Darin P., Chang-Lung Lee, David G. Kirsch, and Cristian T. Badea. “Spectrotemporal CT data acquisition and reconstruction at low dose.” Med Phys 42, no. 11 (November 2015): 6317–36. https://doi.org/10.1118/1.4931407.
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  - Lee, Chang-Lung, Katherine D. Castle, Everett J. Moding, Jordan M. Blum, Nerissa Williams, Lixia Luo, Yan Ma, Luke B. Borst, Yongbaek Kim, and David G. Kirsch. “Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma.” Nat Commun 6 (September 24, 2015): 8477. https://doi.org/10.1038/ncomms9477.
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  - Moding, Everett J., Katherine D. Castle, Bradford A. Perez, Patrick Oh, Hooney D. Min, Hannah Norris, Yan Ma, Diana M. Cardona, Chang-Lung Lee, and David G. Kirsch. “Tumor cells, but not endothelial cells, mediate eradication of primary sarcomas by stereotactic body radiation therapy.” Sci Transl Med 7, no. 278 (March 11, 2015): 278ra34. https://doi.org/10.1126/scitranslmed.aaa4214.
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  - Schönhuber, Nina, Barbara Seidler, Kathleen Schuck, Christian Veltkamp, Christina Schachtler, Magdalena Zukowska, Stefan Eser, et al. “A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.” Nat Med 20, no. 11 (November 2014): 1340–47. https://doi.org/10.1038/nm.3646.
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  - Sachdeva, Mohit, Jeffrey K. Mito, Chang-Lung Lee, Minsi Zhang, Zhizhong Li, Rebecca D. Dodd, David Cason, et al. “MicroRNA-182 drives metastasis of primary sarcomas by targeting multiple genes.” J Clin Invest 124, no. 10 (October 2014): 4305–19. https://doi.org/10.1172/JCI77116.
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  - Moding, Everett J., Chang-Lung Lee, Katherine D. Castle, Patrick Oh, Lan Mao, Shan Zha, Hooney D. Min, Yan Ma, Shiva Das, and David G. Kirsch. “Atm deletion with dual recombinase technology preferentially radiosensitizes tumor endothelium.” J Clin Invest 124, no. 8 (August 2014): 3325–38. https://doi.org/10.1172/JCI73932.
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  - Lee, Chang-Lung, Everett J. Moding, and David G. Kirsch. “Reining in radiation injury: HIF2α in the gut.” Sci Transl Med 6, no. 236 (May 14, 2014): 236fs20. https://doi.org/10.1126/scitranslmed.3009155.
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  - Lee, Chang-Lung, William E. Lento, Katherine D. Castle, Nelson J. Chao, and David G. Kirsch. “Inhibiting glycogen synthase kinase-3 mitigates the hematopoietic acute radiation syndrome in mice.” Radiat Res 181, no. 5 (May 2014): 445–51. https://doi.org/10.1667/RR13692.1.
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  - Lee, Chang-Lung, Hooney Min, Nicholas Befera, Darin Clark, Yi Qi, Shiva Das, G Allan Johnson, Cristian T. Badea, and David G. Kirsch. “Assessing cardiac injury in mice with dual energy-microCT, 4D-microCT, and microSPECT imaging after partial heart irradiation.” Int J Radiat Oncol Biol Phys 88, no. 3 (March 1, 2014): 686–93. https://doi.org/10.1016/j.ijrobp.2013.11.238.
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  - Lee, Chang-Lung, Jordan M. Blum, and David G. Kirsch. “Role of p53 in regulating tissue response to radiation by mechanisms independent of apoptosis.” Transl Cancer Res 2, no. 5 (October 2013): 412–21.
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  - Perez, Bradford A., A Paiman Ghafoori, Chang-Lung Lee, Samuel M. Johnston, Yifan Li, Jacob G. Moroshek, Yan Ma, et al. “Assessing the radiation response of lung cancer with different gene mutations using genetically engineered mice.” Front Oncol 3 (2013): 72. https://doi.org/10.3389/fonc.2013.00072.
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  - Mito, Jeffrey K., Jorge M. Ferrer, Brian E. Brigman, Chang-Lung Lee, Rebecca D. Dodd, William C. Eward, Lisa F. Marshall, et al. “Intraoperative detection and removal of microscopic residual sarcoma using wide-field imaging.” Cancer 118, no. 21 (November 1, 2012): 5320–30. https://doi.org/10.1002/cncr.27458.
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  - Lee, Chang-Lung, Everett J. Moding, Kyle C. Cuneo, Yifan Li, Julie M. Sullivan, Lan Mao, Iman Washington, et al. “p53 functions in endothelial cells to prevent radiation-induced myocardial injury in mice.” Sci Signal 5, no. 234 (July 24, 2012): ra52. https://doi.org/10.1126/scisignal.2002918.
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  - Lee, Chang-Lung, Everett J. Moding, Xiaofang Huang, Yifan Li, Loretta Z. Woodlief, Rafaela C. Rodrigues, Yan Ma, and David G. Kirsch. “Generation of primary tumors with Flp recombinase in FRT-flanked p53 mice.” Dis Model Mech 5, no. 3 (May 2012): 397–402. https://doi.org/10.1242/dmm.009084.
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  - Sullivan, Julie M., Laura B. Jeffords, Chang-Lung Lee, Rafaela Rodrigues, Yan Ma, and David G. Kirsch. “p21 protects "Super p53" mice from the radiation-induced gastrointestinal syndrome.” Radiat Res 177, no. 3 (March 2012): 307–10. https://doi.org/10.1667/rr2545.1.
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  - Lai, Chia-Ping, Chang-Lung Lee, Po-Hsuan Chen, Shu-Hsing Wu, Chien-Chih Yang, and Jei-Fu Shaw. “Molecular analyses of the Arabidopsis TUBBY-like protein gene family.” Plant Physiol 134, no. 4 (April 2004): 1586–97. https://doi.org/10.1104/pp.103.037820.
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- Book Sections
  - Palta, M., C. L. Lee, S. W. Yusuf, and D. G. Kirsch. “Radiation therapy and cardiotoxicity.” In Cardio-Oncology: The Clinical Overlap of Cancer and Heart Disease, 161–74, 2017. https://doi.org/10.1007/978-3-319-43096-6_7.
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- Conference Papers
  - Clark, D. P., C. L. Lee, D. G. Kirsch, and C. T. Badea. “Rank-sparsity constrained, spectro-temporal reconstruction for retrospectively gated, dynamic CT.” In Progress in Biomedical Optics and Imaging Proceedings of Spie, Vol. 9412, 2015. https://doi.org/10.1117/12.2081379.
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Teaching & Mentoring
Recent Courses
- PATHOL 293: Research Independent Study 2022
- PATHOL 793: Research Independent Study 2022

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Education, Training, & Certifications

Previous Appointments & Affiliations

Awards & Honors

Subject Headings

Selected Grants

Fellowships, Supported Research, & Other Grants

Selected Publications

Academic Articles

Book Sections

Conference Papers

Recent Courses