The Fusion Oncogene FUS-CHOP Drives Sarcomagenesis of High-Grade Spindle Cell Sarcomas in Mice.

Journal Article (Journal Article)

Myxoid liposarcoma is a malignant soft tissue sarcoma characterized by a pathognomonic t(12;16)(q13;p11) translocation that produces a fusion oncoprotein, FUS-CHOP. This cancer is remarkably sensitive to radiotherapy and exhibits a unique pattern of extrapulmonary metastasis. Here, we report the generation and characterization of a spatially and temporally restricted mouse model of sarcoma driven by FUS-CHOP. Using different Cre drivers in the adipocyte lineage, we initiated in vivo tumorigenesis by expressing FUS-CHOP in Prrx1+ mesenchymal progenitor cells. In contrast, expression of FUS-CHOP in more differentiated cells does not form tumors in vivo, and early expression of the oncoprotein during embryogenesis is lethal. We also employ in vivo electroporation and CRISPR technology to rapidly generate spatially and temporally restricted mouse models of high-grade FUS-CHOP-driven sarcomas for preclinical studies.

Full Text

Duke Authors

Cited Authors

  • Chen, M; Xu, ES; Leisenring, NH; Cardona, DM; Luo, L; Ma, Y; Ventura, A; Kirsch, DG

Published Date

  • 2019

Published In

Volume / Issue

  • 2019 /

Start / End Page

  • 1340261 -

PubMed ID

  • 31427882

Pubmed Central ID

  • PMC6683777

International Standard Serial Number (ISSN)

  • 1357-714X

Digital Object Identifier (DOI)

  • 10.1155/2019/1340261


  • eng

Conference Location

  • Egypt