Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase.

Book Section

The recognition that nucleic acids can be used as polymeric materials led to the blossoming of the field of DNA nanotechnology, with a broad range of applications in biotechnology, biosensors, diagnostics, and drug delivery. These applications require efficient methods to synthesize and chemically modify high molecular weight DNA. Here, we discuss terminal deoxynucleotidyl transferase (TdT)-catalyzed enzymatic polymerization (TcEP) as an alternative to conventional enzymatic and solid-phase DNA synthesis. We describe biochemical requirements for TcEP and provide step-by-step protocols to carry out TcEP in solution and from surfaces.

Full Text

Duke Authors

Cited Authors

  • Deshpande, S; Yang, Y; Chilkoti, A; Zauscher, S

Published Date

  • January 2019

Volume / Issue

  • 627 /

Start / End Page

  • 163 - 188

PubMed ID

  • 31630739

Digital Object Identifier (DOI)

  • 10.1016/bs.mie.2019.07.044