Topography and pachymetry maps for mouse corneas using optical coherence tomography.

Journal Article (Journal Article)

The majority of the eye's refractive power lies in the cornea, and pathological changes in its shape can affect vision. Small animal models offer an unparalleled degree of control over genetic and environmental factors that can help elucidate mechanisms of diseases affecting corneal shape. However, there is not currently a method to characterize the corneal shape of small animal eyes with topography or pachymetry maps, as is done clinically for humans. We bridge this gap by demonstrating methods using optical coherence tomography (OCT) to generate the first topography and pachymetry (thickness) maps of mouse corneas. Radii of curvature acquired using OCT were validated using calibration spheres as well as in vivo mouse corneas with a mouse keratometer. The resulting topography and pachymetry maps are analogous to those used diagnostically in clinic and potentially allow for characterization of genetically modified mice that replicate key features of human corneal disease.

Full Text

Duke Authors

Cited Authors

  • Liu, AS; Brown, DM; Conn, RE; McNabb, RP; Pardue, MT; Kuo, AN

Published Date

  • January 2020

Published In

Volume / Issue

  • 190 /

Start / End Page

  • 107868 -

PubMed ID

  • 31704241

Pubmed Central ID

  • PMC6961820

Electronic International Standard Serial Number (EISSN)

  • 1096-0007

Digital Object Identifier (DOI)

  • 10.1016/j.exer.2019.107868


  • eng

Conference Location

  • England