In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays.

Journal Article (Review;Journal Article)

"Nonfouling" polymer brush surfaces can greatly improve the performance of in vitro diagnostic (IVD) assays due to the reduction of nonspecific protein adsorption and consequent improvement of signal-to-noise ratios. The development of synthetic polymer brush architectures that suppress adventitious protein adsorption is reviewed, and their integration into surface plasmon resonance and fluorescent sandwich immunoassay formats is discussed. Also, highlighted is a novel, self-contained immunoassay platform (the D4 assay) that transforms time-consuming laboratory-based assays into a user-friendly and point-of-care format with a sensitivity and specificity comparable or better than standard enzyme-linked immunosorbent assay (ELISA) directly from unprocessed samples. These advancements clearly demonstrate the utility of nonfouling polymer brushes as a substrate for ultrasensitive and robust diagnostic assays that may be suitable for clinical testing, in field and laboratory settings.

Full Text

Duke Authors

Cited Authors

  • Heggestad, JT; Fontes, CM; Joh, DY; Hucknall, AM; Chilkoti, A

Published Date

  • January 2020

Published In

Volume / Issue

  • 32 / 2

Start / End Page

  • e1903285 -

PubMed ID

  • 31782843

Pubmed Central ID

  • PMC6986790

Electronic International Standard Serial Number (EISSN)

  • 1521-4095

International Standard Serial Number (ISSN)

  • 0935-9648

Digital Object Identifier (DOI)

  • 10.1002/adma.201903285


  • eng