Super-Resolution Optical Fluctuation Bio-Imaging with Dual-Color Carbon Nanodots.

Published

Journal Article

Success in super-resolution imaging relies on a proper choice of fluorescent probes. Here, we suggest novel easily produced and biocompatible nanoparticles-carbon nanodots-for super-resolution optical fluctuation bioimaging (SOFI). The particles revealed an intrinsic dual-color fluorescence, which corresponds to two subpopulations of particles of different electric charges. The neutral nanoparticles localize to cellular nuclei suggesting their potential use as an inexpensive, easily produced nucleus-specific label. The single particle study revealed that the carbon nanodots possess a unique hybrid combination of fluorescence properties exhibiting characteristics of both dye molecules and semiconductor nanocrystals. The results suggest that charge trapping and redistribution on the surface of the particles triggers their transitions between emissive and dark states. These findings open up new possibilities for the utilization of carbon nanodots in the various super-resolution microscopy methods based on stochastic optical switching.

Full Text

Duke Authors

Cited Authors

  • Chizhik, AM; Stein, S; Dekaliuk, MO; Battle, C; Li, W; Huss, A; Platen, M; Schaap, IAT; Gregor, I; Demchenko, AP; Schmidt, CF; Enderlein, J; Chizhik, AI

Published Date

  • January 2016

Published In

Volume / Issue

  • 16 / 1

Start / End Page

  • 237 - 242

PubMed ID

  • 26605640

Pubmed Central ID

  • 26605640

Electronic International Standard Serial Number (EISSN)

  • 1530-6992

International Standard Serial Number (ISSN)

  • 1530-6992

Digital Object Identifier (DOI)

  • 10.1021/acs.nanolett.5b03609

Language

  • eng