Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.

Journal Article (Journal Article)

While mutagenesis of single genes is now common practice in molecular biology, engineering multiple target genes still requires complex cloning techniques and thus is limited to expert laboratories. Here, we describe "Reiterative Recombination," a user-friendly DNA assembly technique in Saccharomyces cerevisiae for the integration of an indefinite number of DNA fragments sequentially into the yeast genome. The high efficiency of chromosomal integration can further be utilized for the assembly of large combinatorial libraries for metabolic engineering.

Full Text

Duke Authors

Cited Authors

  • Ostrov, N; Wingler, LM; Cornish, VW

Published Date

  • 2013

Published In

Volume / Issue

  • 978 /

Start / End Page

  • 187 - 203

PubMed ID

  • 23423898

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-293-3_14

Language

  • eng

Conference Location

  • United States