Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.
Journal Article (Journal Article)
While mutagenesis of single genes is now common practice in molecular biology, engineering multiple target genes still requires complex cloning techniques and thus is limited to expert laboratories. Here, we describe "Reiterative Recombination," a user-friendly DNA assembly technique in Saccharomyces cerevisiae for the integration of an indefinite number of DNA fragments sequentially into the yeast genome. The high efficiency of chromosomal integration can further be utilized for the assembly of large combinatorial libraries for metabolic engineering.
Full Text
Duke Authors
Cited Authors
- Ostrov, N; Wingler, LM; Cornish, VW
Published Date
- 2013
Published In
Volume / Issue
- 978 /
Start / End Page
- 187 - 203
PubMed ID
- 23423898
Electronic International Standard Serial Number (EISSN)
- 1940-6029
Digital Object Identifier (DOI)
- 10.1007/978-1-62703-293-3_14
Language
- eng
Conference Location
- United States