Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.
Publication
, Journal Article
Ostrov, N; Wingler, LM; Cornish, VW
Published in: Methods Mol Biol
2013
While mutagenesis of single genes is now common practice in molecular biology, engineering multiple target genes still requires complex cloning techniques and thus is limited to expert laboratories. Here, we describe "Reiterative Recombination," a user-friendly DNA assembly technique in Saccharomyces cerevisiae for the integration of an indefinite number of DNA fragments sequentially into the yeast genome. The high efficiency of chromosomal integration can further be utilized for the assembly of large combinatorial libraries for metabolic engineering.
Duke Scholars
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2013
Volume
978
Start / End Page
187 / 203
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae
- Recombination, Genetic
- Metabolic Engineering
- Genetic Engineering
- Developmental Biology
- Cloning, Molecular
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Ostrov, N., Wingler, L. M., & Cornish, V. W. (2013). Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination. Methods Mol Biol, 978, 187–203. https://doi.org/10.1007/978-1-62703-293-3_14
Ostrov, Nili, Laura M. Wingler, and Virginia W. Cornish. “Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.” Methods Mol Biol 978 (2013): 187–203. https://doi.org/10.1007/978-1-62703-293-3_14.
Ostrov N, Wingler LM, Cornish VW. Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination. Methods Mol Biol. 2013;978:187–203.
Ostrov, Nili, et al. “Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.” Methods Mol Biol, vol. 978, 2013, pp. 187–203. Pubmed, doi:10.1007/978-1-62703-293-3_14.
Ostrov N, Wingler LM, Cornish VW. Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination. Methods Mol Biol. 2013;978:187–203.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2013
Volume
978
Start / End Page
187 / 203
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae
- Recombination, Genetic
- Metabolic Engineering
- Genetic Engineering
- Developmental Biology
- Cloning, Molecular
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences