Scholars@Duke

• Background
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  Education, Training, & Certifications

  • Ph.D., Columbia University 2011
• Research
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  Selected Grants

  • Pharmacological Sciences Training Grant awarded by National Institutes of Health 2020 - 2025
  • Molecular Regulation of Cardiovascular 7 TM Receptors awarded by National Institutes of Health 1976 - 2023
  • Nanobodies for the detection of CCR2 activation awarded by Washington University in St. Louis 2021 - 2022
  • Beta2-adrenergic receptor mutagenesis for studies of biased agonism awarded by National Institutes of Health 2014 - 2015
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  External Relationships

  • Third Rock Ventures (GPCRco)
• Publications & Artistic Works
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  Selected Publications

  • Academic Articles

    • Wellner, Alon, Conor McMahon, Morgan S. A. Gilman, Jonathan R. Clements, Sarah Clark, Kianna M. Nguyen, Ming H. Ho, et al. “Rapid generation of potent antibodies by autonomous hypermutation in yeast.” Nat Chem Biol, June 24, 2021. https://doi.org/10.1038/s41589-021-00832-4.
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    • Wellner, Alon, Conor McMahon, Morgan S. A. Gilman, Jonathan R. Clements, Sarah Clark, Kianna M. Nguyen, Ming H. Ho, et al. “Rapid generation of potent antibodies by autonomous hypermutation in yeast.” Biorxiv, November 11, 2020. https://doi.org/10.1101/2020.11.11.378778.
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    • Wingler, Laura M., and Robert J. Lefkowitz. “Conformational Basis of G Protein-Coupled Receptor Signaling Versatility.” Trends Cell Biol 30, no. 9 (September 2020): 736–47. https://doi.org/10.1016/j.tcb.2020.06.002.
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    • McMahon, Conor, Dean P. Staus, Laura M. Wingler, Jialu Wang, Meredith A. Skiba, Matthias Elgeti, Wayne L. Hubbell, Howard A. Rockman, Andrew C. Kruse, and Robert J. Lefkowitz. “Synthetic nanobodies as angiotensin receptor blockers.” Proc Natl Acad Sci U S A 117, no. 33 (August 18, 2020): 20284–91. https://doi.org/10.1073/pnas.2009029117.
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    • Manglik, Aashish, Laura M. Wingler, Howard A. Rockman, and Robert J. Lefkowitz. “β-Arrestin-Biased Angiotensin II Receptor Agonists for COVID-19.” Circulation 142, no. 4 (July 2020): 318–20. https://doi.org/10.1161/circulationaha.120.048723.
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    • Staus, Dean P., Hongli Hu, Michael J. Robertson, Alissa L. W. Kleinhenz, Laura M. Wingler, William D. Capel, Naomi R. Latorraca, Robert J. Lefkowitz, and Georgios Skiniotis. “Structure of the M2 muscarinic receptor-β-arrestin complex in a lipid nanodisc.” Nature 579, no. 7798 (March 2020): 297–302. https://doi.org/10.1038/s41586-020-1954-0.
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    • Suomivuori, Carl-Mikael, Naomi R. Latorraca, Laura M. Wingler, Stephan Eismann, Matthew C. King, Alissa L. W. Kleinhenz, Meredith A. Skiba, et al. “Molecular mechanism of biased signaling in a prototypical G protein-coupled receptor.” Science 367, no. 6480 (February 21, 2020): 881–87. https://doi.org/10.1126/science.aaz0326.
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    • Wingler, Laura M., Meredith A. Skiba, Conor McMahon, Dean P. Staus, Alissa L. W. Kleinhenz, Carl-Mikael Suomivuori, Naomi R. Latorraca, Ron O. Dror, Robert J. Lefkowitz, and Andrew C. Kruse. “Angiotensin and biased analogs induce structurally distinct active conformations within a GPCR.” Science 367, no. 6480 (February 21, 2020): 888–92. https://doi.org/10.1126/science.aay9813.
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    • Staus, Dean P., Laura M. Wingler, Dmitry Pichugin, Robert Scott Prosser, and Robert J. Lefkowitz. “Detergent- and phospholipid-based reconstitution systems have differential effects on constitutive activity of G-protein-coupled receptors.” J Biol Chem 294, no. 36 (September 6, 2019): 13218–23. https://doi.org/10.1074/jbc.AC119.009848.
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    • Wingler, Laura M., Conor McMahon, Dean P. Staus, Robert J. Lefkowitz, and Andrew C. Kruse. “Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody.” Cell 176, no. 3 (January 24, 2019): 479-490.e12. https://doi.org/10.1016/j.cell.2018.12.006.
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    • Wingler, Laura M., Matthias Elgeti, Daniel Hilger, Naomi R. Latorraca, Michael T. Lerch, Dean P. Staus, Ron O. Dror, Brian K. Kobilka, Wayne L. Hubbell, and Robert J. Lefkowitz. “Angiotensin Analogs with Divergent Bias Stabilize Distinct Receptor Conformations.” Cell 176, no. 3 (January 24, 2019): 468-478.e11. https://doi.org/10.1016/j.cell.2018.12.005.
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    • Choi, Minjung, Dean P. Staus, Laura M. Wingler, Seungkirl Ahn, Biswaranjan Pani, William D. Capel, and Robert J. Lefkowitz. “G protein-coupled receptor kinases (GRKs) orchestrate biased agonism at the β2-adrenergic receptor.” Sci Signal 11, no. 544 (August 21, 2018). https://doi.org/10.1126/scisignal.aar7084.
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    • Ahn, Seungkirl, Biswaranjan Pani, Alem W. Kahsai, Eva K. Olsen, Gitte Husemoen, Mikkel Vestergaard, Lei Jin, et al. “Small-Molecule Positive Allosteric Modulators of the β2-Adrenoceptor Isolated from DNA-Encoded Libraries.” Mol Pharmacol 94, no. 2 (August 2018): 850–61. https://doi.org/10.1124/mol.118.111948.
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    • Staus, Dean P., Laura M. Wingler, Minjung Choi, Biswaranjan Pani, Aashish Manglik, Andrew C. Kruse, and Robert J. Lefkowitz. “Sortase ligation enables homogeneous GPCR phosphorylation to reveal diversity in β-arrestin coupling.” Proc Natl Acad Sci U S A 115, no. 15 (April 10, 2018): 3834–39. https://doi.org/10.1073/pnas.1722336115.
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    • Paek, Jaeho, Marian Kalocsay, Dean P. Staus, Laura Wingler, Roberta Pascolutti, Joao A. Paulo, Steven P. Gygi, and Andrew C. Kruse. “Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling.” Cell 169, no. 2 (April 6, 2017): 338-349.e11. https://doi.org/10.1016/j.cell.2017.03.028.
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    • Ahn, Seungkirl, Alem W. Kahsai, Biswaranjan Pani, Qin-Ting Wang, Shuai Zhao, Alissa L. Wall, Ryan T. Strachan, et al. “Allosteric "beta-blocker" isolated from a DNA-encoded small molecule library.” Proc Natl Acad Sci U S A 114, no. 7 (February 14, 2017): 1708–13. https://doi.org/10.1073/pnas.1620645114.
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    • Kahsai, Alem W., James W. Wisler, Jungmin Lee, Seungkirl Ahn, Thomas J. Cahill Iii, S Moses Dennison, Dean P. Staus, et al. “Conformationally selective RNA aptamers allosterically modulate the β2-adrenoceptor.” Nat Chem Biol 12, no. 9 (September 2016): 709–16. https://doi.org/10.1038/nchembio.2126.
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    • Staus, Dean P., Ryan T. Strachan, Aashish Manglik, Biswaranjan Pani, Alem W. Kahsai, Tae Hun Kim, Laura M. Wingler, et al. “Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation.” Nature 535, no. 7612 (July 21, 2016): 448–52. https://doi.org/10.1038/nature18636.
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    • Staus, Dean P., Laura M. Wingler, Ryan T. Strachan, Soren G. F. Rasmussen, Els Pardon, Seungkirl Ahn, Jan Steyaert, Brian K. Kobilka, and Robert J. Lefkowitz. “Regulation of β2-adrenergic receptor function by conformationally selective single-domain intrabodies.” Mol Pharmacol 85, no. 3 (March 2014): 472–81. https://doi.org/10.1124/mol.113.089516.
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    • Harton, Marie D., Laura M. Wingler, and Virginia W. Cornish. “Transcriptional regulation improves the throughput of three-hybrid counter selections in Saccharomyces cerevisiae.” Biotechnol J 8, no. 12 (December 2013): 1485–91. https://doi.org/10.1002/biot.201300186.
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    • Aristotelous, Tonia, Seungkirl Ahn, Arun K. Shukla, Sylwia Gawron, Maria F. Sassano, Alem W. Kahsai, Laura M. Wingler, et al. “Discovery of β2 Adrenergic Receptor Ligands Using Biosensor Fragment Screening of Tagged Wild-Type Receptor.” Acs Med Chem Lett 4, no. 10 (October 10, 2013): 1005–10. https://doi.org/10.1021/ml400312j.
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    • Ostrov, Nili, Laura M. Wingler, and Virginia W. Cornish. “Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.” Methods Mol Biol 978 (2013): 187–203. https://doi.org/10.1007/978-1-62703-293-3_14.
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    • Wingler, Laura M., and Virginia W. Cornish. “Reiterative Recombination for the in vivo assembly of libraries of multigene pathways.” Proc Natl Acad Sci U S A 108, no. 37 (September 13, 2011): 15135–40. https://doi.org/10.1073/pnas.1100507108.
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    • Wingler, Laura M., and Virginia W. Cornish. “A library approach for the discovery of customized yeast three-hybrid counter selections.” Chembiochem 12, no. 5 (March 21, 2011): 715–17. https://doi.org/10.1002/cbic.201000543.
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  • Conference Papers

    • McMahon, Conor, Laura Wingler, Dean Staus, Robert Lefkowitz, and Andrew Kruse. “So Long Llamas: Structure of Activated Angiotensin Receptor Stabilized by a Synthetic Nanobody.” In The Faseb Journal, 34:1–1. Wiley, 2020. https://doi.org/10.1096/fasebj.2020.34.s1.08990.
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    • Suomivuori, Carl-Mikael, Naomi R. Latorraca, Laura M. Wingler, Stephan Eismann, Matthew C. King, Alissa L. W. Kleinhenz, Meredith A. Skiba, et al. “Molecular Mechanism of Biased Signaling in a Prototypical G-proteincoupled Receptor.” In Biophysical Journal, 118:162A-162A. CELL PRESS, 2020.
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    • Pfeiffer, Conrad T., Clarice Gareri, Joao A. Paulo, Dean P. Staus, Laura M. Wingler, Steven P. Gygi, and Howard A. Rockman. “Mechanoactivation of Angiotensin II Type 1 Receptor Induces a Unique Phosphorylation "Barcode" Compared to Activation by Angiotensin II and [beta]-Arrestin Biased Ligand, TRV120023.” In Circulation, Vol. 138, 2018.
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    • Gareri, Clarice, Joao A. Paulo, Alem W. Kashai, Jialu Wang, Kenji Hanada, Dean P. Staus, Laura M. Wingler, Steven P. Gygi, and Howard A. Rockman. “Mechanical Stretch Activates Biased AT1R Signaling Through a Unique B-arrestin Conformation.” In Circulation, Vol. 136. LIPPINCOTT WILLIAMS & WILKINS, 2017.
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    • Choi, Minjung, Dean Staus, Laura Wingler, and Robert Lefkowitz. “beta 2-Adrenergic receptor mutant results in functional bias towards G-protein mediated signaling pathways and reveals the underappreciated role of GRKs in biased agonism.” In Abstracts of Papers of the American Chemical Society, Vol. 252, 2016.
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    • Rein, Lindsay A. M., Minyong Chen, Barbara S. Theriot, James W. Wisler, Laura M. Wingler, Richard T. Premont, Wei Chen, Julia K. L. Walker, and Robert J. Lefkowitz. “Targeting β-arrestin2 Enhances Survival in a Murine Model of Chronic Myeloid Leukemia.” In Blood, 122:857–857. American Society of Hematology, 2013. https://doi.org/10.1182/blood.v122.21.857.857.
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• Teaching & Mentoring
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  Recent Courses

  • CMB 710D: Cell & Molecular Biology Module IV 2021

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