A near-infrared genetically encoded calcium indicator for in vivo imaging.

Journal Article (Journal Article)

While calcium imaging has become a mainstay of modern neuroscience, the spectral properties of current fluorescent calcium indicators limit deep-tissue imaging as well as simultaneous use with other probes. Using two monomeric near-infrared (NIR) fluorescent proteins (FPs), we engineered an NIR Förster resonance energy transfer (FRET)-based genetically encoded calcium indicator (iGECI). iGECI exhibits high levels of brightness and photostability and an increase up to 600% in the fluorescence response to calcium. In dissociated neurons, iGECI detects spontaneous neuronal activity and electrically and optogenetically induced firing. We validated the performance of iGECI up to a depth of almost 400 µm in acute brain slices using one-photon light-sheet imaging. Applying hybrid photoacoustic and fluorescence microscopy, we simultaneously monitored neuronal and hemodynamic activities in the mouse brain through an intact skull, with resolutions of ~3 μm (lateral) and ~25-50 μm (axial). Using two-photon imaging, we detected evoked and spontaneous neuronal activity in the mouse visual cortex, with fluorescence changes of up to 25%. iGECI allows biosensors and optogenetic actuators to be multiplexed without spectral crosstalk.

Full Text

Duke Authors

Cited Authors

  • Shemetov, AA; Monakhov, MV; Zhang, Q; Canton-Josh, JE; Kumar, M; Chen, M; Matlashov, ME; Li, X; Yang, W; Nie, L; Shcherbakova, DM; Kozorovitskiy, Y; Yao, J; Ji, N; Verkhusha, VV

Published Date

  • March 2021

Published In

Volume / Issue

  • 39 / 3

Start / End Page

  • 368 - 377

PubMed ID

  • 33106681

Pubmed Central ID

  • PMC7956128

Electronic International Standard Serial Number (EISSN)

  • 1546-1696

Digital Object Identifier (DOI)

  • 10.1038/s41587-020-0710-1


  • eng

Conference Location

  • United States