Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media.
There are currently no effective treatments to regenerate the heart after cardiac injury. Following cardiac injury, heart muscle cells, also known as cardiomyocytes, die in large numbers. The adult mammalian heart does not have the ability to replace these dead cardiomyocytes. In their place, fibroblasts invade the injury zone and generate a scar. The scar impairs cardiac function. An important approach to cardiac regeneration is direct cardiac reprogramming, whereby cardiac fibroblasts within the scar are directly converted into functional cardiomyocytes. Several laboratories have achieved direct cardiac reprogramming via overexpression of the cardiac transcription factors. In contrast, we utilize a combination of four miRNAs (miR-1, miR-133, miR-208, miR-499) that we call miR Combo. One common issue regarding direct cardiac reprogramming strategies is low efficiency. Recently, we have demonstrated that the efficiency of direct cardiac reprogramming is enhanced in the chemically defined reprogramming media.
Duke Scholars
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Related Subject Headings
- Transfection
- Selenium
- Regeneration
- Real-Time Polymerase Chain Reaction
- Myocytes, Cardiac
- MicroRNAs
- Mice
- Fluorescent Antibody Technique
- Flow Cytometry
- Fibroblasts
Citation
Published In
DOI
EISSN
Publication Date
Volume
Start / End Page
Location
Related Subject Headings
- Transfection
- Selenium
- Regeneration
- Real-Time Polymerase Chain Reaction
- Myocytes, Cardiac
- MicroRNAs
- Mice
- Fluorescent Antibody Technique
- Flow Cytometry
- Fibroblasts