Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution.

Journal Article (Journal Article)

Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.

Full Text

Duke Authors

Cited Authors

  • Ding, Y; Lee, J; Ma, J; Sung, K; Yokota, T; Singh, N; Dooraghi, M; Abiri, P; Wang, Y; Kulkarni, RP; Nakano, A; Nguyen, TP; Fei, P; Hsiai, TK

Published Date

  • February 6, 2017

Published In

Volume / Issue

  • 7 /

Start / End Page

  • 42209 -

PubMed ID

  • 28165052

Pubmed Central ID

  • PMC5292685

Electronic International Standard Serial Number (EISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/srep42209

Language

  • eng

Conference Location

  • England