Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution.
Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.
Duke Scholars
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Related Subject Headings
- Proteins
- Potassium Channels
- Myocardium
- Microscopy, Fluorescence
- Mice
- Imaging, Three-Dimensional
- Heart Ventricles
- Green Fluorescent Proteins
- Fluorescence
- Cell Lineage
Citation
Published In
DOI
EISSN
Publication Date
Volume
Start / End Page
Location
Related Subject Headings
- Proteins
- Potassium Channels
- Myocardium
- Microscopy, Fluorescence
- Mice
- Imaging, Three-Dimensional
- Heart Ventricles
- Green Fluorescent Proteins
- Fluorescence
- Cell Lineage