Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.

Journal Article (Journal Article)

Expression of transgenes within a single generation by direct DNA injection into vertebrate embryos has been plagued by inefficient and nonuniform gene expression. We report a novel strategy for efficient and stable expression of transgenes driven by both ubiquitous and tissue-specific promoters by direct DNA injection into developing Xenopus laevis embryos. This strategy involves flanking expression cassettes of interest with inverted terminal repeat sequences (ITRs) from adeno-associated virus. Our results suggest that the ITR strategy may be generally applicable to other systems, such as zebra fish and embryonic stem cells, and may enable tissue-specific expression of transgenes in problematic contexts.

Full Text

Duke Authors

Cited Authors

  • Fu, Y; Wang, Y; Evans, SM

Published Date

  • March 1, 1998

Published In

Volume / Issue

  • 16 / 3

Start / End Page

  • 253 - 257

PubMed ID

  • 9528004

International Standard Serial Number (ISSN)

  • 1087-0156

Digital Object Identifier (DOI)

  • 10.1038/nbt0398-253

Language

  • eng

Conference Location

  • United States