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Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.

Publication ,  Journal Article
Fu, Y; Wang, Y; Evans, SM
Published in: Nat Biotechnol
March 1998

Expression of transgenes within a single generation by direct DNA injection into vertebrate embryos has been plagued by inefficient and nonuniform gene expression. We report a novel strategy for efficient and stable expression of transgenes driven by both ubiquitous and tissue-specific promoters by direct DNA injection into developing Xenopus laevis embryos. This strategy involves flanking expression cassettes of interest with inverted terminal repeat sequences (ITRs) from adeno-associated virus. Our results suggest that the ITR strategy may be generally applicable to other systems, such as zebra fish and embryonic stem cells, and may enable tissue-specific expression of transgenes in problematic contexts.

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Published In

Nat Biotechnol

DOI

ISSN

1087-0156

Publication Date

March 1998

Volume

16

Issue

3

Start / End Page

253 / 257

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Xenopus laevis
  • Transgenes
  • Somites
  • Repetitive Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Plasmids
  • Organ Specificity
  • Luminescent Proteins
  • Heart
 

Citation

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Fu, Y., Wang, Y., & Evans, S. M. (1998). Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. Nat Biotechnol, 16(3), 253–257. https://doi.org/10.1038/nbt0398-253
Fu, Y., Y. Wang, and S. M. Evans. “Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.Nat Biotechnol 16, no. 3 (March 1998): 253–57. https://doi.org/10.1038/nbt0398-253.
Fu Y, Wang Y, Evans SM. Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. Nat Biotechnol. 1998 Mar;16(3):253–7.
Fu, Y., et al. “Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.Nat Biotechnol, vol. 16, no. 3, Mar. 1998, pp. 253–57. Pubmed, doi:10.1038/nbt0398-253.
Fu Y, Wang Y, Evans SM. Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. Nat Biotechnol. 1998 Mar;16(3):253–257.

Published In

Nat Biotechnol

DOI

ISSN

1087-0156

Publication Date

March 1998

Volume

16

Issue

3

Start / End Page

253 / 257

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Xenopus laevis
  • Transgenes
  • Somites
  • Repetitive Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Plasmids
  • Organ Specificity
  • Luminescent Proteins
  • Heart