Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.
Publication
, Journal Article
Fu, Y; Wang, Y; Evans, SM
Published in: Nat Biotechnol
March 1998
Expression of transgenes within a single generation by direct DNA injection into vertebrate embryos has been plagued by inefficient and nonuniform gene expression. We report a novel strategy for efficient and stable expression of transgenes driven by both ubiquitous and tissue-specific promoters by direct DNA injection into developing Xenopus laevis embryos. This strategy involves flanking expression cassettes of interest with inverted terminal repeat sequences (ITRs) from adeno-associated virus. Our results suggest that the ITR strategy may be generally applicable to other systems, such as zebra fish and embryonic stem cells, and may enable tissue-specific expression of transgenes in problematic contexts.
Duke Scholars
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Published In
Nat Biotechnol
DOI
ISSN
1087-0156
Publication Date
March 1998
Volume
16
Issue
3
Start / End Page
253 / 257
Location
United States
Related Subject Headings
- beta-Galactosidase
- Xenopus laevis
- Transgenes
- Somites
- Repetitive Sequences, Nucleic Acid
- Promoter Regions, Genetic
- Plasmids
- Organ Specificity
- Luminescent Proteins
- Heart
Citation
APA
Chicago
ICMJE
MLA
NLM
Fu, Y., Wang, Y., & Evans, S. M. (1998). Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. Nat Biotechnol, 16(3), 253–257. https://doi.org/10.1038/nbt0398-253
Fu, Y., Y. Wang, and S. M. Evans. “Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.” Nat Biotechnol 16, no. 3 (March 1998): 253–57. https://doi.org/10.1038/nbt0398-253.
Fu Y, Wang Y, Evans SM. Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. Nat Biotechnol. 1998 Mar;16(3):253–7.
Fu, Y., et al. “Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.” Nat Biotechnol, vol. 16, no. 3, Mar. 1998, pp. 253–57. Pubmed, doi:10.1038/nbt0398-253.
Fu Y, Wang Y, Evans SM. Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. Nat Biotechnol. 1998 Mar;16(3):253–257.
Published In
Nat Biotechnol
DOI
ISSN
1087-0156
Publication Date
March 1998
Volume
16
Issue
3
Start / End Page
253 / 257
Location
United States
Related Subject Headings
- beta-Galactosidase
- Xenopus laevis
- Transgenes
- Somites
- Repetitive Sequences, Nucleic Acid
- Promoter Regions, Genetic
- Plasmids
- Organ Specificity
- Luminescent Proteins
- Heart