Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.

Journal Article (Journal Article)

The functional and molecular similarities and distinctions between human and murine astrocytes are poorly understood. Here, we report the development of an immunopanning method to acutely purify astrocytes from fetal, juvenile, and adult human brains and to maintain these cells in serum-free cultures. We found that human astrocytes have abilities similar to those of murine astrocytes in promoting neuronal survival, inducing functional synapse formation, and engulfing synaptosomes. In contrast to existing observations in mice, we found that mature human astrocytes respond robustly to glutamate. Next, we performed RNA sequencing of healthy human astrocytes along with astrocytes from epileptic and tumor foci and compared these to human neurons, oligodendrocytes, microglia, and endothelial cells (available at http://www.brainrnaseq.org). With these profiles, we identified novel human-specific astrocyte genes and discovered a transcriptome-wide transformation between astrocyte precursor cells and mature post-mitotic astrocytes. These data represent some of the first cell-type-specific molecular profiles of the healthy and diseased human brain.

Full Text

Duke Authors

Cited Authors

  • Zhang, Y; Sloan, SA; Clarke, LE; Caneda, C; Plaza, CA; Blumenthal, PD; Vogel, H; Steinberg, GK; Edwards, MSB; Li, G; Duncan, JA; Cheshier, SH; Shuer, LM; Chang, EF; Grant, GA; Gephart, MGH; Barres, BA

Published Date

  • January 6, 2016

Published In

Volume / Issue

  • 89 / 1

Start / End Page

  • 37 - 53

PubMed ID

  • 26687838

Pubmed Central ID

  • PMC4707064

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2015.11.013

Language

  • eng

Conference Location

  • United States