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Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.

Publication ,  Journal Article
Zhang, Y; Sloan, SA; Clarke, LE; Caneda, C; Plaza, CA; Blumenthal, PD; Vogel, H; Steinberg, GK; Edwards, MSB; Li, G; Duncan, JA; Cheshier, SH ...
Published in: Neuron
January 6, 2016

The functional and molecular similarities and distinctions between human and murine astrocytes are poorly understood. Here, we report the development of an immunopanning method to acutely purify astrocytes from fetal, juvenile, and adult human brains and to maintain these cells in serum-free cultures. We found that human astrocytes have abilities similar to those of murine astrocytes in promoting neuronal survival, inducing functional synapse formation, and engulfing synaptosomes. In contrast to existing observations in mice, we found that mature human astrocytes respond robustly to glutamate. Next, we performed RNA sequencing of healthy human astrocytes along with astrocytes from epileptic and tumor foci and compared these to human neurons, oligodendrocytes, microglia, and endothelial cells (available at http://www.brainrnaseq.org). With these profiles, we identified novel human-specific astrocyte genes and discovered a transcriptome-wide transformation between astrocyte precursor cells and mature post-mitotic astrocytes. These data represent some of the first cell-type-specific molecular profiles of the healthy and diseased human brain.

Duke Scholars

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Published In

Neuron

DOI

EISSN

1097-4199

Publication Date

January 6, 2016

Volume

89

Issue

1

Start / End Page

37 / 53

Location

United States

Related Subject Headings

  • Transcriptome
  • Stem Cells
  • Oligodendroglia
  • Neurons
  • Neurology & Neurosurgery
  • Microglia
  • Mice
  • Humans
  • Cells, Cultured
  • Cell Survival
 

Citation

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Chicago
ICMJE
MLA
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Zhang, Y., Sloan, S. A., Clarke, L. E., Caneda, C., Plaza, C. A., Blumenthal, P. D., … Barres, B. A. (2016). Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. Neuron, 89(1), 37–53. https://doi.org/10.1016/j.neuron.2015.11.013
Zhang, Ye, Steven A. Sloan, Laura E. Clarke, Christine Caneda, Colton A. Plaza, Paul D. Blumenthal, Hannes Vogel, et al. “Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.Neuron 89, no. 1 (January 6, 2016): 37–53. https://doi.org/10.1016/j.neuron.2015.11.013.
Zhang Y, Sloan SA, Clarke LE, Caneda C, Plaza CA, Blumenthal PD, et al. Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. Neuron. 2016 Jan 6;89(1):37–53.
Zhang, Ye, et al. “Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.Neuron, vol. 89, no. 1, Jan. 2016, pp. 37–53. Pubmed, doi:10.1016/j.neuron.2015.11.013.
Zhang Y, Sloan SA, Clarke LE, Caneda C, Plaza CA, Blumenthal PD, Vogel H, Steinberg GK, Edwards MSB, Li G, Duncan JA, Cheshier SH, Shuer LM, Chang EF, Grant GA, Gephart MGH, Barres BA. Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. Neuron. 2016 Jan 6;89(1):37–53.
Journal cover image

Published In

Neuron

DOI

EISSN

1097-4199

Publication Date

January 6, 2016

Volume

89

Issue

1

Start / End Page

37 / 53

Location

United States

Related Subject Headings

  • Transcriptome
  • Stem Cells
  • Oligodendroglia
  • Neurons
  • Neurology & Neurosurgery
  • Microglia
  • Mice
  • Humans
  • Cells, Cultured
  • Cell Survival