Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.
Publication
, Journal Article
Oliinyk, OS; Ma, C; Pletnev, S; Baloban, M; Taboada, C; Sheng, H; Yao, J; Verkhusha, VV
Published in: Nat Methods
January 2023
Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
January 2023
Volume
20
Issue
1
Start / End Page
70 / 74
Location
United States
Related Subject Headings
- Optical Imaging
- Mice
- Mammals
- Fluorescent Dyes
- Developmental Biology
- Animals
- 31 Biological sciences
- 11 Medical and Health Sciences
- 10 Technology
- 06 Biological Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Oliinyk, O. S., Ma, C., Pletnev, S., Baloban, M., Taboada, C., Sheng, H., … Verkhusha, V. V. (2023). Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein. Nat Methods, 20(1), 70–74. https://doi.org/10.1038/s41592-022-01683-0
Oliinyk, Olena S., Chenshuo Ma, Sergei Pletnev, Mikhail Baloban, Carlos Taboada, Huaxin Sheng, Junjie Yao, and Vladislav V. Verkhusha. “Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.” Nat Methods 20, no. 1 (January 2023): 70–74. https://doi.org/10.1038/s41592-022-01683-0.
Oliinyk OS, Ma C, Pletnev S, Baloban M, Taboada C, Sheng H, et al. Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein. Nat Methods. 2023 Jan;20(1):70–4.
Oliinyk, Olena S., et al. “Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.” Nat Methods, vol. 20, no. 1, Jan. 2023, pp. 70–74. Pubmed, doi:10.1038/s41592-022-01683-0.
Oliinyk OS, Ma C, Pletnev S, Baloban M, Taboada C, Sheng H, Yao J, Verkhusha VV. Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein. Nat Methods. 2023 Jan;20(1):70–74.
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
January 2023
Volume
20
Issue
1
Start / End Page
70 / 74
Location
United States
Related Subject Headings
- Optical Imaging
- Mice
- Mammals
- Fluorescent Dyes
- Developmental Biology
- Animals
- 31 Biological sciences
- 11 Medical and Health Sciences
- 10 Technology
- 06 Biological Sciences