Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.

Journal Article (Journal Article)

Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.

Full Text

Duke Authors

Cited Authors

  • Oliinyk, OS; Ma, C; Pletnev, S; Baloban, M; Taboada, C; Sheng, H; Yao, J; Verkhusha, VV

Published Date

  • January 2023

Published In

Volume / Issue

  • 20 / 1

Start / End Page

  • 70 - 74

PubMed ID

  • 36456785

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

Digital Object Identifier (DOI)

  • 10.1038/s41592-022-01683-0


  • eng

Conference Location

  • United States