Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction.

Journal Article (Journal Article)

Previous studies have shown the implication of beta-defensins in host defense of the human body. The human beta-defensins 1 and 2 (hBD-1, hBD-2) have been isolated by biochemical methods. Here we report the identification of a third human beta-defensin, called human beta-defensin 3 (hBD-3; cDNA sequence, Genbank accession no. AF295370), based on bioinformatics and functional genomic analysis. Expression of hBD-3 is detected throughout epithelia of many organs and in non-epithelial tissues. In contrast to hBD-2, which is upregulated by microorganisms or tumor necrosis factor-alpha (TNF-alpha), hBD-3 expression is increased particularly after stimulation by interferon-gamma. Synthetic hBD-3 exhibits a strong antimicrobial activity against gram-negative and gram-positive bacteria and fungi, including Burkholderia cepacia. In addition, hBD-3 activates monocytes and elicits ion channel activity in biomembranes, specifically in oocytes of Xenopus laevis. This paper also shows that screening of genomic sequences is a valuable tool with which to identify novel regulatory peptides. Human beta-defensins represent a family of antimicrobial peptides differentially expressed in most tissues, regulated by specific mechanisms, and exerting physiological functions not only related to direct host defense.

Full Text

Duke Authors

Cited Authors

  • García, JR; Jaumann, F; Schulz, S; Krause, A; Rodríguez-Jiménez, J; Forssmann, U; Adermann, K; Klüver, E; Vogelmeier, C; Becker, D; Hedrich, R; Forssmann, WG; Bals, R

Published Date

  • November 2001

Published In

Volume / Issue

  • 306 / 2

Start / End Page

  • 257 - 264

PubMed ID

  • 11702237

International Standard Serial Number (ISSN)

  • 0302-766X

Digital Object Identifier (DOI)

  • 10.1007/s004410100433

Language

  • eng

Conference Location

  • Germany