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Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction.

Publication ,  Journal Article
García, JR; Jaumann, F; Schulz, S; Krause, A; Rodríguez-Jiménez, J; Forssmann, U; Adermann, K; Klüver, E; Vogelmeier, C; Becker, D; Hedrich, R ...
Published in: Cell Tissue Res
November 2001

Previous studies have shown the implication of beta-defensins in host defense of the human body. The human beta-defensins 1 and 2 (hBD-1, hBD-2) have been isolated by biochemical methods. Here we report the identification of a third human beta-defensin, called human beta-defensin 3 (hBD-3; cDNA sequence, Genbank accession no. AF295370), based on bioinformatics and functional genomic analysis. Expression of hBD-3 is detected throughout epithelia of many organs and in non-epithelial tissues. In contrast to hBD-2, which is upregulated by microorganisms or tumor necrosis factor-alpha (TNF-alpha), hBD-3 expression is increased particularly after stimulation by interferon-gamma. Synthetic hBD-3 exhibits a strong antimicrobial activity against gram-negative and gram-positive bacteria and fungi, including Burkholderia cepacia. In addition, hBD-3 activates monocytes and elicits ion channel activity in biomembranes, specifically in oocytes of Xenopus laevis. This paper also shows that screening of genomic sequences is a valuable tool with which to identify novel regulatory peptides. Human beta-defensins represent a family of antimicrobial peptides differentially expressed in most tissues, regulated by specific mechanisms, and exerting physiological functions not only related to direct host defense.

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Published In

Cell Tissue Res

DOI

ISSN

0302-766X

Publication Date

November 2001

Volume

306

Issue

2

Start / End Page

257 / 264

Location

Germany

Related Subject Headings

  • beta-Defensins
  • Xenopus laevis
  • Sequence Alignment
  • Patch-Clamp Techniques
  • Oocytes
  • Neurology & Neurosurgery
  • Monocytes
  • Molecular Sequence Data
  • Macrophages
  • Ion Channels
 

Citation

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García, J. R., Jaumann, F., Schulz, S., Krause, A., Rodríguez-Jiménez, J., Forssmann, U., … Bals, R. (2001). Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res, 306(2), 257–264. https://doi.org/10.1007/s004410100433
García, J. R., F. Jaumann, S. Schulz, A. Krause, J. Rodríguez-Jiménez, U. Forssmann, K. Adermann, et al. “Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction.Cell Tissue Res 306, no. 2 (November 2001): 257–64. https://doi.org/10.1007/s004410100433.
García JR, Jaumann F, Schulz S, Krause A, Rodríguez-Jiménez J, Forssmann U, Adermann K, Klüver E, Vogelmeier C, Becker D, Hedrich R, Forssmann WG, Bals R. Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res. 2001 Nov;306(2):257–264.
Journal cover image

Published In

Cell Tissue Res

DOI

ISSN

0302-766X

Publication Date

November 2001

Volume

306

Issue

2

Start / End Page

257 / 264

Location

Germany

Related Subject Headings

  • beta-Defensins
  • Xenopus laevis
  • Sequence Alignment
  • Patch-Clamp Techniques
  • Oocytes
  • Neurology & Neurosurgery
  • Monocytes
  • Molecular Sequence Data
  • Macrophages
  • Ion Channels