Programmable synthetic biomolecular condensates for cellular control.

Journal Article (Journal Article)

The formation of biomolecular condensates mediated by a coupling of associative and segregative phase transitions plays a critical role in controlling diverse cellular functions in nature. This has inspired the use of phase transitions to design synthetic systems. While design rules of phase transitions have been established for many synthetic intrinsically disordered proteins, most efforts have focused on investigating their phase behaviors in a test tube. Here, we present a rational engineering approach to program the formation and physical properties of synthetic condensates to achieve intended cellular functions. We demonstrate this approach through targeted plasmid sequestration and transcription regulation in bacteria and modulation of a protein circuit in mammalian cells. Our approach lays the foundation for engineering designer condensates for synthetic biology applications.

Full Text

Duke Authors

Cited Authors

  • Dai, Y; Farag, M; Lee, D; Zeng, X; Kim, K; Son, H-I; Guo, X; Su, J; Peterson, N; Mohammed, J; Ney, M; Shapiro, DM; Pappu, RV; Chilkoti, A; You, L

Published Date

  • April 2023

Published In

Volume / Issue

  • 19 / 4

Start / End Page

  • 518 - 528

PubMed ID

  • 36747054

Electronic International Standard Serial Number (EISSN)

  • 1552-4469

International Standard Serial Number (ISSN)

  • 1552-4450

Digital Object Identifier (DOI)

  • 10.1038/s41589-022-01252-8


  • eng