Transplant approach establishes that kidneys are responsible for serum CSF-1 but require a stimulus in MRL-lpr mice.

Colony stimulating factor-1 (CSF-1) is a chemoattractant and growth factor for macrophages. In autoimmune MRL-lpr mice, CSF-1 is detected in the circulation and there is an increase in CSF-1 transcripts and macrophages in the kidney. The purpose of this study was to establish whether the MRL-lpr kidney is responsible for increasing serum CSF-1, and to determine if the expression of CSF-1 requires a circulating component in MRL-lpr mice. We transplanted a MRL-lpr kidney with nephritis [serum CSF-1 = 32.6 +/- 5.1 colony forming units (CFU)] into a bilaterally nephrectomized normal MRL-++ recipient. Circulating CSF-1 increased from 1.2 +/- CFU to 14.1 +/_ 5.6 CFU in recipients by one week. Continuous production of CSF-1 required a stimulating component since: (1) CSF-1 in the kidney and circulation disappeared several weeks after engraftment into MRL-++ mice and (2) isolated glomeruli from MRL-lpr required stimulation to express CSF-1. In the transplanted MRL-lpr kidney, hypercellularity in the glomerulus and interstitium (predominantly macrophages) returned to normal as rapidly as two weeks after engraftment into MRL-++. Thus, this study establishes that the kidney is responsible for circulating CSF-1 in MRL-lpr mice. Without the autoimmune environment CSF-1, macrophages, but not T cells, disappear and nephritis resolves.

Duke Scholars

Author Thomas Myron Coffman Medicine, Nephrology