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Transplant approach establishes that kidneys are responsible for serum CSF-1 but require a stimulus in MRL-lpr mice.

Publication ,  Journal Article
Naito, T; Griffiths, RC; Coffman, TM; Kelley, VR
Published in: Kidney Int
January 1996

Colony stimulating factor-1 (CSF-1) is a chemoattractant and growth factor for macrophages. In autoimmune MRL-lpr mice, CSF-1 is detected in the circulation and there is an increase in CSF-1 transcripts and macrophages in the kidney. The purpose of this study was to establish whether the MRL-lpr kidney is responsible for increasing serum CSF-1, and to determine if the expression of CSF-1 requires a circulating component in MRL-lpr mice. We transplanted a MRL-lpr kidney with nephritis [serum CSF-1 = 32.6 +/- 5.1 colony forming units (CFU)] into a bilaterally nephrectomized normal MRL-++ recipient. Circulating CSF-1 increased from 1.2 +/- CFU to 14.1 +/_ 5.6 CFU in recipients by one week. Continuous production of CSF-1 required a stimulating component since: (1) CSF-1 in the kidney and circulation disappeared several weeks after engraftment into MRL-++ mice and (2) isolated glomeruli from MRL-lpr required stimulation to express CSF-1. In the transplanted MRL-lpr kidney, hypercellularity in the glomerulus and interstitium (predominantly macrophages) returned to normal as rapidly as two weeks after engraftment into MRL-++. Thus, this study establishes that the kidney is responsible for circulating CSF-1 in MRL-lpr mice. Without the autoimmune environment CSF-1, macrophages, but not T cells, disappear and nephritis resolves.

Duke Scholars

Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

January 1996

Volume

49

Issue

1

Start / End Page

67 / 74

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Nephritis
  • Nephrectomy
  • Mice
  • Male
  • Macrophages
  • Macrophage Colony-Stimulating Factor
  • Lipopolysaccharides
  • Kidney Transplantation
  • Kidney Glomerulus
 

Citation

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MLA
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Naito, T., Griffiths, R. C., Coffman, T. M., & Kelley, V. R. (1996). Transplant approach establishes that kidneys are responsible for serum CSF-1 but require a stimulus in MRL-lpr mice. Kidney Int, 49(1), 67–74. https://doi.org/10.1038/ki.1996.9
Naito, T., R. C. Griffiths, T. M. Coffman, and V. R. Kelley. “Transplant approach establishes that kidneys are responsible for serum CSF-1 but require a stimulus in MRL-lpr mice.Kidney Int 49, no. 1 (January 1996): 67–74. https://doi.org/10.1038/ki.1996.9.
Naito T, Griffiths RC, Coffman TM, Kelley VR. Transplant approach establishes that kidneys are responsible for serum CSF-1 but require a stimulus in MRL-lpr mice. Kidney Int. 1996 Jan;49(1):67–74.
Naito, T., et al. “Transplant approach establishes that kidneys are responsible for serum CSF-1 but require a stimulus in MRL-lpr mice.Kidney Int, vol. 49, no. 1, Jan. 1996, pp. 67–74. Pubmed, doi:10.1038/ki.1996.9.
Naito T, Griffiths RC, Coffman TM, Kelley VR. Transplant approach establishes that kidneys are responsible for serum CSF-1 but require a stimulus in MRL-lpr mice. Kidney Int. 1996 Jan;49(1):67–74.
Journal cover image

Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

January 1996

Volume

49

Issue

1

Start / End Page

67 / 74

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Nephritis
  • Nephrectomy
  • Mice
  • Male
  • Macrophages
  • Macrophage Colony-Stimulating Factor
  • Lipopolysaccharides
  • Kidney Transplantation
  • Kidney Glomerulus