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Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans.

Publication ,  Journal Article
Görlach, J; Fox, DS; Cutler, NS; Cox, GM; Perfect, JR; Heitman, J
Published in: EMBO J
July 17, 2000

Calcineurin is the conserved target of the immunosuppressants cyclosporin A and FK506. Using the yeast two-hybrid system, we identified a novel calcineurin binding protein, CBP1, from the pathogenic fungus Cryptococcus neoformans. We show that CBP1 binds to calcineurin in vitro and in vivo, and FKBP12-FK506 inhibits CBP1 binding to calcineurin. Cryptococcus neoformans cbp1 mutant strains exhibit modest defects in growth under stress conditions and virulence, similar to but less severe than the phenotypes of calcineurin mutants. Saccharomyces cerevisiae mutants lacking the CBP1 homolog RCN1 are, like calcineurin mutants, sensitive to lithium cation stress. CBP1 shares a central peptide sequence motif, SPPxSPP, with related proteins in S.CEREVISIAE:, Schizosaccharomyces pombe, Drosophila melanogaster, Caenorhabditis elegans and humans, and peptides containing this motif altered calcineurin activity in vitro. Interestingly, the human CBP1 homolog DSCR1 is encoded by the Down's syndrome candidate region interval on chromosome 21, is highly expressed in the heart and central nervous system, and may play a role in calcineurin functions in heart development, neurite extension and memory.

Duke Scholars

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

July 17, 2000

Volume

19

Issue

14

Start / End Page

3618 / 3629

Location

England

Related Subject Headings

  • Virulence
  • Two-Hybrid System Techniques
  • Tacrolimus Binding Proteins
  • Sequence Alignment
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Protein Binding
  • Phenotype
  • Mutation
  • Molecular Sequence Data
 

Citation

APA
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Görlach, J., Fox, D. S., Cutler, N. S., Cox, G. M., Perfect, J. R., & Heitman, J. (2000). Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans. EMBO J, 19(14), 3618–3629. https://doi.org/10.1093/emboj/19.14.3618
Görlach, J., D. S. Fox, N. S. Cutler, G. M. Cox, J. R. Perfect, and J. Heitman. “Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans.EMBO J 19, no. 14 (July 17, 2000): 3618–29. https://doi.org/10.1093/emboj/19.14.3618.
Görlach J, Fox DS, Cutler NS, Cox GM, Perfect JR, Heitman J. Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans. EMBO J. 2000 Jul 17;19(14):3618–29.
Görlach, J., et al. “Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans.EMBO J, vol. 19, no. 14, July 2000, pp. 3618–29. Pubmed, doi:10.1093/emboj/19.14.3618.
Görlach J, Fox DS, Cutler NS, Cox GM, Perfect JR, Heitman J. Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans. EMBO J. 2000 Jul 17;19(14):3618–3629.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

July 17, 2000

Volume

19

Issue

14

Start / End Page

3618 / 3629

Location

England

Related Subject Headings

  • Virulence
  • Two-Hybrid System Techniques
  • Tacrolimus Binding Proteins
  • Sequence Alignment
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Protein Binding
  • Phenotype
  • Mutation
  • Molecular Sequence Data