BRCA2 monoclonal antibodies react with differentiating epithelium.

Published

Journal Article

The BRCA2 gene has previously been suggested to play a role in proliferation and DNA repair. Germline mutations in the BRCA2 gene predispose individuals to early onset, hereditary breast cancer. To better understand the expression pattern and function of the BRCA2 gene product, we have developed immunological reagents specific for BRCA2. These reagents recognize full-length (384 kDa) recombinant human BRCA2 proteins in transfected cell lysates as well as multiple smaller recombinant BRCA2 polypeptides. Detection of native BRCA2 protein in most tissue types, including breast epithelium, requires sensitive techniques such as immunoprecipitation-Western blot analysis. However, we have demonstrated strong reactivity of our immunological reagents with differentiating epithelium, including epidermis, thymic epithelium, and squamous cell carcinoma. These data suggest that BRCA2 may play a role in processes associated with cellular differentiation, in addition to its previously suggested roles in proliferation and DNA repair.

Full Text

Duke Authors

Cited Authors

  • Gilliam, LK; Lobenhofer, EK; Greer, PK; Scearce, RM; Cirisano, FD; Marks, JR; Hale, LP

Published Date

  • August 2002

Published In

Volume / Issue

  • 21 / 4

Start / End Page

  • 261 - 269

PubMed ID

  • 12193279

Pubmed Central ID

  • 12193279

International Standard Serial Number (ISSN)

  • 1536-8599

Digital Object Identifier (DOI)

  • 10.1089/153685902760213877

Language

  • eng

Conference Location

  • United States