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BRCA2 monoclonal antibodies react with differentiating epithelium.

Publication ,  Journal Article
Gilliam, LK; Lobenhofer, EK; Greer, PK; Scearce, RM; Cirisano, FD; Marks, JR; Hale, LP
Published in: Hybrid Hybridomics
August 2002

The BRCA2 gene has previously been suggested to play a role in proliferation and DNA repair. Germline mutations in the BRCA2 gene predispose individuals to early onset, hereditary breast cancer. To better understand the expression pattern and function of the BRCA2 gene product, we have developed immunological reagents specific for BRCA2. These reagents recognize full-length (384 kDa) recombinant human BRCA2 proteins in transfected cell lysates as well as multiple smaller recombinant BRCA2 polypeptides. Detection of native BRCA2 protein in most tissue types, including breast epithelium, requires sensitive techniques such as immunoprecipitation-Western blot analysis. However, we have demonstrated strong reactivity of our immunological reagents with differentiating epithelium, including epidermis, thymic epithelium, and squamous cell carcinoma. These data suggest that BRCA2 may play a role in processes associated with cellular differentiation, in addition to its previously suggested roles in proliferation and DNA repair.

Duke Scholars

Published In

Hybrid Hybridomics

DOI

ISSN

1536-8599

Publication Date

August 2002

Volume

21

Issue

4

Start / End Page

261 / 269

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Recombinant Proteins
  • Ovarian Neoplasms
  • Mice
  • Keratinocytes
  • Immunology
  • Humans
  • Female
  • Epithelial Cells
  • Cell Differentiation
 

Citation

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Gilliam, L. K., Lobenhofer, E. K., Greer, P. K., Scearce, R. M., Cirisano, F. D., Marks, J. R., & Hale, L. P. (2002). BRCA2 monoclonal antibodies react with differentiating epithelium. Hybrid Hybridomics, 21(4), 261–269. https://doi.org/10.1089/153685902760213877
Gilliam, Lisa K., Edward K. Lobenhofer, Paula K. Greer, Richard M. Scearce, Frank D. Cirisano, Jeffrey R. Marks, and Laura P. Hale. “BRCA2 monoclonal antibodies react with differentiating epithelium.Hybrid Hybridomics 21, no. 4 (August 2002): 261–69. https://doi.org/10.1089/153685902760213877.
Gilliam LK, Lobenhofer EK, Greer PK, Scearce RM, Cirisano FD, Marks JR, et al. BRCA2 monoclonal antibodies react with differentiating epithelium. Hybrid Hybridomics. 2002 Aug;21(4):261–9.
Gilliam, Lisa K., et al. “BRCA2 monoclonal antibodies react with differentiating epithelium.Hybrid Hybridomics, vol. 21, no. 4, Aug. 2002, pp. 261–69. Pubmed, doi:10.1089/153685902760213877.
Gilliam LK, Lobenhofer EK, Greer PK, Scearce RM, Cirisano FD, Marks JR, Hale LP. BRCA2 monoclonal antibodies react with differentiating epithelium. Hybrid Hybridomics. 2002 Aug;21(4):261–269.

Published In

Hybrid Hybridomics

DOI

ISSN

1536-8599

Publication Date

August 2002

Volume

21

Issue

4

Start / End Page

261 / 269

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Recombinant Proteins
  • Ovarian Neoplasms
  • Mice
  • Keratinocytes
  • Immunology
  • Humans
  • Female
  • Epithelial Cells
  • Cell Differentiation