Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold.

Journal Article (Journal Article)

This paper describes a simple strategy to biochemically manipulate a surface at the nanoscale by enzyme dip-pen nanolithography using an endonuclease (DNase I) that is directly patterned on a self-assembled monolayer presenting a terminal oligonucleotide. Physisorbed nanopatterns of DNase I carried out nanoscale enzymology at the surface creating oligonucleotide patterns with the fidelity of the patterned enzyme because of the affinity of the enzyme for the immobilized, oligonucleotide substrate.

Full Text

Duke Authors

Cited Authors

  • Hyun, J; Kim, J; Craig, SL; Chilkoti, A

Published Date

  • April 2004

Published In

Volume / Issue

  • 126 / 15

Start / End Page

  • 4770 - 4771

PubMed ID

  • 15080668

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja049956q


  • eng