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Biochemical evidence for enhanced sensitivity to N-methyl-D-aspartate in the hippocampal formation of kindled rats.

Publication ,  Journal Article
Morrisett, RA; Chow, C; Nadler, JV; McNamara, JO
Published in: Brain Res
September 4, 1989

The inhibitory effect of N-methyl-D-aspartate (NMDA) upon carbachol-stimulated phosphoinositide (PI) hydrolysis was studied in transverse hippocampal slices prepared from control and amygdaloid kindled rats. Kindling significantly increased the inhibitory effect of NMDA (10 microM) in slices prepared from animals 24 h after the last class 5 kindled seizure, resulting in a steepening of the dose-response curve for NMDA. The enhanced sensitivity to NMDA was long-lasting in that it was also present in slices prepared from animals sacrificed 28-35 days after the last class 5 seizure. The increased sensitivity to NMDA was selective in that inhibition of carbachol-stimulated PI hydrolysis by kainic acid or phorbol-12,13-diacetate was not different in control and kindled animals. Neither NMDA, kainic acid, phorbol ester nor carbachol alone had any significantly different effects in slices from kindled versus control animals. These data demonstrate a selective and enhanced sensitivity of the kindled hippocampus to NMDA. This enhanced sensitivity to the principal class of excitatory neurotransmitter may be one mechanism underlying the development and maintenance of kindled epilepsy.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

September 4, 1989

Volume

496

Issue

1-2

Start / End Page

25 / 28

Location

Netherlands

Related Subject Headings

  • Rats, Inbred Strains
  • Rats
  • Phosphatidylinositols
  • Neurology & Neurosurgery
  • N-Methylaspartate
  • Male
  • Kindling, Neurologic
  • In Vitro Techniques
  • Hippocampus
  • Aspartic Acid
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Morrisett, R. A., Chow, C., Nadler, J. V., & McNamara, J. O. (1989). Biochemical evidence for enhanced sensitivity to N-methyl-D-aspartate in the hippocampal formation of kindled rats. Brain Res, 496(1–2), 25–28. https://doi.org/10.1016/0006-8993(89)91048-2
Morrisett, R. A., C. Chow, J. V. Nadler, and J. O. McNamara. “Biochemical evidence for enhanced sensitivity to N-methyl-D-aspartate in the hippocampal formation of kindled rats.Brain Res 496, no. 1–2 (September 4, 1989): 25–28. https://doi.org/10.1016/0006-8993(89)91048-2.
Morrisett RA, Chow C, Nadler JV, McNamara JO. Biochemical evidence for enhanced sensitivity to N-methyl-D-aspartate in the hippocampal formation of kindled rats. Brain Res. 1989 Sep 4;496(1–2):25–8.
Morrisett, R. A., et al. “Biochemical evidence for enhanced sensitivity to N-methyl-D-aspartate in the hippocampal formation of kindled rats.Brain Res, vol. 496, no. 1–2, Sept. 1989, pp. 25–28. Pubmed, doi:10.1016/0006-8993(89)91048-2.
Morrisett RA, Chow C, Nadler JV, McNamara JO. Biochemical evidence for enhanced sensitivity to N-methyl-D-aspartate in the hippocampal formation of kindled rats. Brain Res. 1989 Sep 4;496(1–2):25–28.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

September 4, 1989

Volume

496

Issue

1-2

Start / End Page

25 / 28

Location

Netherlands

Related Subject Headings

  • Rats, Inbred Strains
  • Rats
  • Phosphatidylinositols
  • Neurology & Neurosurgery
  • N-Methylaspartate
  • Male
  • Kindling, Neurologic
  • In Vitro Techniques
  • Hippocampus
  • Aspartic Acid