A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia.

Published

Journal Article

We describe a simple and robust flow cytometry assay for ZAP-70 and CD38 expression. The steps required to validate this assay in a clinical flow cytometry laboratory are described. Two criteria were used to characterize ZAP-70 expression into positive, negative, and indeterminate categories and applied to 111 cases of chronic lymphocytic leukemia (CLL) resulting in 29.7% positive, 56.8% negative, and 13.5% indeterminate cases. A sensitivity-specificity crossover plot between ZAP-70 and CD38 suggested a cutoff of 12.5% for defining CD38 positivity. ZAP-70+ cases were significantly more likely to be at a higher clinical stage and, together with CD38+ cases, were more likely to have unmutated IgV(H). However, for individual patients, the concordance between these markers was not perfect. It may be necessary to evaluate several prognostic markers simultaneously in CLL, and availability of convenient assays for ZAP-70 and CD38 is desirable for optimal clinical decision making.

Full Text

Duke Authors

Cited Authors

  • Hassanein, NM; Perkinson, KR; Alcancia, F; Goodman, BK; Weinberg, JB; Lagoo, AS

Published Date

  • May 2010

Published In

Volume / Issue

  • 133 / 5

Start / End Page

  • 708 - 717

PubMed ID

  • 20395517

Pubmed Central ID

  • 20395517

Electronic International Standard Serial Number (EISSN)

  • 1943-7722

International Standard Serial Number (ISSN)

  • 0002-9173

Digital Object Identifier (DOI)

  • 10.1309/ajcpqs4oxjjsz5kn

Language

  • eng