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A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia.

Publication ,  Journal Article
Hassanein, NM; Perkinson, KR; Alcancia, F; Goodman, BK; Weinberg, JB; Lagoo, AS
Published in: Am J Clin Pathol
May 2010

We describe a simple and robust flow cytometry assay for ZAP-70 and CD38 expression. The steps required to validate this assay in a clinical flow cytometry laboratory are described. Two criteria were used to characterize ZAP-70 expression into positive, negative, and indeterminate categories and applied to 111 cases of chronic lymphocytic leukemia (CLL) resulting in 29.7% positive, 56.8% negative, and 13.5% indeterminate cases. A sensitivity-specificity crossover plot between ZAP-70 and CD38 suggested a cutoff of 12.5% for defining CD38 positivity. ZAP-70+ cases were significantly more likely to be at a higher clinical stage and, together with CD38+ cases, were more likely to have unmutated IgV(H). However, for individual patients, the concordance between these markers was not perfect. It may be necessary to evaluate several prognostic markers simultaneously in CLL, and availability of convenient assays for ZAP-70 and CD38 is desirable for optimal clinical decision making.

Duke Scholars

Published In

Am J Clin Pathol

DOI

EISSN

1943-7722

Publication Date

May 2010

Volume

133

Issue

5

Start / End Page

708 / 717

Location

England

Related Subject Headings

  • ZAP-70 Protein-Tyrosine Kinase
  • Prognosis
  • Predictive Value of Tests
  • Pathology
  • Neoplasm Staging
  • Membrane Glycoproteins
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hassanein, N. M., Perkinson, K. R., Alcancia, F., Goodman, B. K., Weinberg, J. B., & Lagoo, A. S. (2010). A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia. Am J Clin Pathol, 133(5), 708–717. https://doi.org/10.1309/AJCPQS4OXJJSZ5KN
Hassanein, Nagwa M., Kathryn R. Perkinson, Felisa Alcancia, Barbara K. Goodman, J Brice Weinberg, and Anand S. Lagoo. “A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia.Am J Clin Pathol 133, no. 5 (May 2010): 708–17. https://doi.org/10.1309/AJCPQS4OXJJSZ5KN.
Hassanein NM, Perkinson KR, Alcancia F, Goodman BK, Weinberg JB, Lagoo AS. A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia. Am J Clin Pathol. 2010 May;133(5):708–17.
Hassanein, Nagwa M., et al. “A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia.Am J Clin Pathol, vol. 133, no. 5, May 2010, pp. 708–17. Pubmed, doi:10.1309/AJCPQS4OXJJSZ5KN.
Hassanein NM, Perkinson KR, Alcancia F, Goodman BK, Weinberg JB, Lagoo AS. A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia. Am J Clin Pathol. 2010 May;133(5):708–717.

Published In

Am J Clin Pathol

DOI

EISSN

1943-7722

Publication Date

May 2010

Volume

133

Issue

5

Start / End Page

708 / 717

Location

England

Related Subject Headings

  • ZAP-70 Protein-Tyrosine Kinase
  • Prognosis
  • Predictive Value of Tests
  • Pathology
  • Neoplasm Staging
  • Membrane Glycoproteins
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Humans