Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis.

Published

Journal Article

A successful vaccine vector for human immunodeficiency virus type 1 (HIV-1) should induce anti-HIV-1 immune responses at mucosal sites. We have generated recombinant Mycobacterium smegmatis vectors that express the HIV-1 group M consensus envelope protein (Env) as a surface, intracellular, or secreted protein and have tested them in animals for induction of both anti-HIV-1 T-cell and antibody responses. Recombinant M. smegmatis engineered for expression of secreted protein induced optimal T-cell gamma interferon enzyme-linked immunospot assay responses to HIV-1 envelope in the spleen, female reproductive tract, and lungs. Unlike with the induction of T-cell responses, priming and boosting with recombinant M. smegmatis did not induce anti-HIV-1 envelope antibody responses, due primarily to insufficient protein expression of the insert. However, immunization with recombinant M. smegmatis expressing HIV-1 Env was able to prime for an HIV-1 Env protein boost for the induction of anti-HIV-1 antibody responses.

Full Text

Duke Authors

Cited Authors

  • Yu, J-S; Peacock, JW; Vanleeuwen, S; Hsu, T; Jacobs, WR; Cayabyab, MJ; Letvin, NL; Frothingham, R; Staats, HF; Liao, H-X; Haynes, BF

Published Date

  • November 2006

Published In

Volume / Issue

  • 13 / 11

Start / End Page

  • 1204 - 1211

PubMed ID

  • 16943347

Pubmed Central ID

  • 16943347

Electronic International Standard Serial Number (EISSN)

  • 1556-679X

International Standard Serial Number (ISSN)

  • 1556-6811

Digital Object Identifier (DOI)

  • 10.1128/CVI.00195-06

Language

  • eng