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Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials.

Publication ,  Journal Article
Huang, X; Zauscher, S; Klitzman, B; Truskey, GA; Reichert, WM; Kenan, DJ; Grinstaff, MW
Published in: Ann Biomed Eng
June 2010

Resorbable scaffolds such as polyglycolic acid (PGA) are employed in a number of clinical and tissue engineering applications owing to their desirable property of allowing remodeling to form native tissue over time. However, native PGA does not promote endothelial cell adhesion. Here we describe a novel treatment with hetero-bifunctional peptide linkers, termed "interfacial biomaterials" (IFBMs), which are used to alter the surface of PGA to provide appropriate biological cues. IFBMs couple an affinity peptide for the material with a biologically active peptide that promotes desired cellular responses. One such PGA affinity peptide was coupled to the integrin binding domain, Arg-Gly-Asp (RGD), to build a chemically synthesized bimodular 27 amino acid peptide that mediated interactions between PGA and integrin receptors on endothelial cells. Quartz crystal microbalance with dissipation monitoring (QCMD) was used to determine the association constant (K (A) 1 x 10(7) M(-1)) and surface thickness (~3.5 nm). Cell binding studies indicated that IFBM efficiently mediated adhesion, spreading, and cytoskeletal organization of endothelial cells on PGA in an integrin-dependent manner. We show that the IFBM peptide promotes a 200% increase in endothelial cell binding to PGA as well as 70-120% increase in cell spreading from 30 to 60 minutes after plating.

Duke Scholars

Published In

Ann Biomed Eng

DOI

EISSN

1573-9686

Publication Date

June 2010

Volume

38

Issue

6

Start / End Page

1965 / 1976

Location

United States

Related Subject Headings

  • Surface Properties
  • Polyglycolic Acid
  • Oligopeptides
  • Materials Testing
  • Humans
  • Endothelial Cells
  • Coated Materials, Biocompatible
  • Cells, Cultured
  • Cell Movement
  • Cell Adhesion
 

Citation

APA
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ICMJE
MLA
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Huang, X., Zauscher, S., Klitzman, B., Truskey, G. A., Reichert, W. M., Kenan, D. J., & Grinstaff, M. W. (2010). Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials. Ann Biomed Eng, 38(6), 1965–1976. https://doi.org/10.1007/s10439-010-9986-5
Huang, Xin, Stefan Zauscher, Bruce Klitzman, George A. Truskey, William M. Reichert, Daniel J. Kenan, and Mark W. Grinstaff. “Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials.Ann Biomed Eng 38, no. 6 (June 2010): 1965–76. https://doi.org/10.1007/s10439-010-9986-5.
Huang X, Zauscher S, Klitzman B, Truskey GA, Reichert WM, Kenan DJ, et al. Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials. Ann Biomed Eng. 2010 Jun;38(6):1965–76.
Huang, Xin, et al. “Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials.Ann Biomed Eng, vol. 38, no. 6, June 2010, pp. 1965–76. Pubmed, doi:10.1007/s10439-010-9986-5.
Huang X, Zauscher S, Klitzman B, Truskey GA, Reichert WM, Kenan DJ, Grinstaff MW. Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials. Ann Biomed Eng. 2010 Jun;38(6):1965–1976.
Journal cover image

Published In

Ann Biomed Eng

DOI

EISSN

1573-9686

Publication Date

June 2010

Volume

38

Issue

6

Start / End Page

1965 / 1976

Location

United States

Related Subject Headings

  • Surface Properties
  • Polyglycolic Acid
  • Oligopeptides
  • Materials Testing
  • Humans
  • Endothelial Cells
  • Coated Materials, Biocompatible
  • Cells, Cultured
  • Cell Movement
  • Cell Adhesion