An ear punch model for studying the effect of radiation on wound healing.

Journal Article

PURPOSE: Radiation and wound combined injury represents a major clinical challenge because of the synergistic interactions that lead to higher morbidity and mortality than either insult would produce singly. The purpose of this study was to develop a mouse ear punch model to study the physiological mechanisms underlying radiation effects on healing wounds. MATERIALS AND METHODS: Surgical wounds were induced by a 2 mm surgical punch in the ear pinnae of MRL/MpJ mice. Photographs of the wounds were taken and the sizes of the ear punch wounds were quantified by image analysis. Local radiation to the ear was delivered by orthovoltage X-ray irradiator using a specially constructed jig that shields the other parts of body. RESULTS: Using this model, we demonstrated that local radiation to the wound area significantly delayed the healing of ear punch wounds in a dose-dependent fashion. The addition of sublethal whole body irradiation (7 Gy) further delayed the healing of ear punch wounds. These results were replicated in C57BL/6 mice; however, wound healing in MRL/MpJ mice was accelerated. CONCLUSIONS: These data indicate that the mouse ear punch model is a valuable model to study radiation and wound combined injury.

Full Text

Duke Authors

Cited Authors

  • Deoliveira, D; Jiao, Y; Ross, JR; Corbin, K; Xiao, Q; Toncheva, G; Anderson-Evans, C; Yoshizumi, TT; Chen, BJ; Chao, NJ

Published Date

  • August 2011

Published In

Volume / Issue

  • 87 / 8

Start / End Page

  • 869 - 877

PubMed ID

  • 21480768

Electronic International Standard Serial Number (EISSN)

  • 1362-3095

Digital Object Identifier (DOI)

  • 10.3109/09553002.2011.568575

Language

  • eng

Conference Location

  • England