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An ear punch model for studying the effect of radiation on wound healing.

Publication ,  Journal Article
Deoliveira, D; Jiao, Y; Ross, JR; Corbin, K; Xiao, Q; Toncheva, G; Anderson-Evans, C; Yoshizumi, TT; Chen, BJ; Chao, NJ
Published in: Int J Radiat Biol
August 2011

PURPOSE: Radiation and wound combined injury represents a major clinical challenge because of the synergistic interactions that lead to higher morbidity and mortality than either insult would produce singly. The purpose of this study was to develop a mouse ear punch model to study the physiological mechanisms underlying radiation effects on healing wounds. MATERIALS AND METHODS: Surgical wounds were induced by a 2 mm surgical punch in the ear pinnae of MRL/MpJ mice. Photographs of the wounds were taken and the sizes of the ear punch wounds were quantified by image analysis. Local radiation to the ear was delivered by orthovoltage X-ray irradiator using a specially constructed jig that shields the other parts of body. RESULTS: Using this model, we demonstrated that local radiation to the wound area significantly delayed the healing of ear punch wounds in a dose-dependent fashion. The addition of sublethal whole body irradiation (7 Gy) further delayed the healing of ear punch wounds. These results were replicated in C57BL/6 mice; however, wound healing in MRL/MpJ mice was accelerated. CONCLUSIONS: These data indicate that the mouse ear punch model is a valuable model to study radiation and wound combined injury.

Duke Scholars

Published In

Int J Radiat Biol

DOI

EISSN

1362-3095

Publication Date

August 2011

Volume

87

Issue

8

Start / End Page

869 / 877

Location

England

Related Subject Headings

  • Wounds, Penetrating
  • Wound Healing
  • Radiation Dosage
  • Oncology & Carcinogenesis
  • Mice, Inbred C57BL
  • Mice
  • Female
  • Ear
  • Disease Models, Animal
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Deoliveira, D., Jiao, Y., Ross, J. R., Corbin, K., Xiao, Q., Toncheva, G., … Chao, N. J. (2011). An ear punch model for studying the effect of radiation on wound healing. Int J Radiat Biol, 87(8), 869–877. https://doi.org/10.3109/09553002.2011.568575
Deoliveira, Divino, Yiqun Jiao, Joel R. Ross, Kayla Corbin, Qizhen Xiao, Greta Toncheva, Colin Anderson-Evans, Terry T. Yoshizumi, Benny J. Chen, and Nelson J. Chao. “An ear punch model for studying the effect of radiation on wound healing.Int J Radiat Biol 87, no. 8 (August 2011): 869–77. https://doi.org/10.3109/09553002.2011.568575.
Deoliveira D, Jiao Y, Ross JR, Corbin K, Xiao Q, Toncheva G, et al. An ear punch model for studying the effect of radiation on wound healing. Int J Radiat Biol. 2011 Aug;87(8):869–77.
Deoliveira, Divino, et al. “An ear punch model for studying the effect of radiation on wound healing.Int J Radiat Biol, vol. 87, no. 8, Aug. 2011, pp. 869–77. Pubmed, doi:10.3109/09553002.2011.568575.
Deoliveira D, Jiao Y, Ross JR, Corbin K, Xiao Q, Toncheva G, Anderson-Evans C, Yoshizumi TT, Chen BJ, Chao NJ. An ear punch model for studying the effect of radiation on wound healing. Int J Radiat Biol. 2011 Aug;87(8):869–877.

Published In

Int J Radiat Biol

DOI

EISSN

1362-3095

Publication Date

August 2011

Volume

87

Issue

8

Start / End Page

869 / 877

Location

England

Related Subject Headings

  • Wounds, Penetrating
  • Wound Healing
  • Radiation Dosage
  • Oncology & Carcinogenesis
  • Mice, Inbred C57BL
  • Mice
  • Female
  • Ear
  • Disease Models, Animal
  • Animals