Enzyme redesign guided by cancer-derived IDH1 mutations.
Publication
, Journal Article
Reitman, ZJ; Choi, BD; Spasojevic, I; Bigner, DD; Sampson, JH; Yan, H
Published in: Nat Chem Biol
November 2012
Mutations in an enzyme can result in a neomorphic catalytic activity in cancers. We applied cancer-associated mutations from isocitrate dehydrogenases to homologous residues in the active sites of homoisocitrate dehydrogenases to derive enzymes that catalyze the conversion of 2-oxoadipate to (R)-2-hydroxyadipate, a critical step for adipic acid production. Thus, we provide a prototypic example of how insights from cancer genome sequencing and functional studies can aid in enzyme redesign.
Duke Scholars
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Published In
Nat Chem Biol
DOI
EISSN
1552-4469
Publication Date
November 2012
Volume
8
Issue
11
Start / End Page
887 / 889
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae
- Protein Engineering
- Neoplasms
- Mutation
- Models, Molecular
- Isocitrate Dehydrogenase
- Humans
- Catalytic Domain
- Biochemistry & Molecular Biology
- Biocatalysis
Citation
APA
Chicago
ICMJE
MLA
NLM
Reitman, Z. J., Choi, B. D., Spasojevic, I., Bigner, D. D., Sampson, J. H., & Yan, H. (2012). Enzyme redesign guided by cancer-derived IDH1 mutations. Nat Chem Biol, 8(11), 887–889. https://doi.org/10.1038/nchembio.1065
Reitman, Zachary J., Bryan D. Choi, Ivan Spasojevic, Darell D. Bigner, John H. Sampson, and Hai Yan. “Enzyme redesign guided by cancer-derived IDH1 mutations.” Nat Chem Biol 8, no. 11 (November 2012): 887–89. https://doi.org/10.1038/nchembio.1065.
Reitman ZJ, Choi BD, Spasojevic I, Bigner DD, Sampson JH, Yan H. Enzyme redesign guided by cancer-derived IDH1 mutations. Nat Chem Biol. 2012 Nov;8(11):887–9.
Reitman, Zachary J., et al. “Enzyme redesign guided by cancer-derived IDH1 mutations.” Nat Chem Biol, vol. 8, no. 11, Nov. 2012, pp. 887–89. Pubmed, doi:10.1038/nchembio.1065.
Reitman ZJ, Choi BD, Spasojevic I, Bigner DD, Sampson JH, Yan H. Enzyme redesign guided by cancer-derived IDH1 mutations. Nat Chem Biol. 2012 Nov;8(11):887–889.
Published In
Nat Chem Biol
DOI
EISSN
1552-4469
Publication Date
November 2012
Volume
8
Issue
11
Start / End Page
887 / 889
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae
- Protein Engineering
- Neoplasms
- Mutation
- Models, Molecular
- Isocitrate Dehydrogenase
- Humans
- Catalytic Domain
- Biochemistry & Molecular Biology
- Biocatalysis