Distinctive gene transduction efficiencies of commonly used viral vectors in the retina.

Published

Journal Article

The transduction efficiency and cell tropism of viral vectors rAAV2/1, rAAV2, Ad5, Ad5/F35, and Lentivirus were evaluated in retina. All viral vectors achieved efficient transduction in living rat retina. However, each vector showed distinctive efficiency in vitro especially for rAAV2/1, which displayed poor transduction in cultured retinal cells. Distinctive cell-specific GFP expression was observed in vivo and in vitro for the same viral vector. The cell-specific tropism was not strictly correlated with the correspondent distribution of viral receptors in retina. These results provided important insights into the selection of appropriate vectors when specific retinal diseases are considered for gene therapy.

Full Text

Duke Authors

Cited Authors

  • Zhang, S-H; Wu, J-H; Wu, X-B; Dong, X-Y; Liu, X-J; Li, C-Y; Qian-Huang,

Published Date

  • January 2008

Published In

Volume / Issue

  • 33 / 1

Start / End Page

  • 81 - 90

PubMed ID

  • 18214745

Pubmed Central ID

  • 18214745

International Standard Serial Number (ISSN)

  • 0271-3683

Digital Object Identifier (DOI)

  • 10.1080/02713680701799408

Language

  • eng

Conference Location

  • England