Skip to main content
Journal cover image

Distinctive gene transduction efficiencies of commonly used viral vectors in the retina.

Publication ,  Journal Article
Zhang, S-H; Wu, J-H; Wu, X-B; Dong, X-Y; Liu, X-J; Li, C-Y; Qian-Huang
Published in: Curr Eye Res
January 2008

The transduction efficiency and cell tropism of viral vectors rAAV2/1, rAAV2, Ad5, Ad5/F35, and Lentivirus were evaluated in retina. All viral vectors achieved efficient transduction in living rat retina. However, each vector showed distinctive efficiency in vitro especially for rAAV2/1, which displayed poor transduction in cultured retinal cells. Distinctive cell-specific GFP expression was observed in vivo and in vitro for the same viral vector. The cell-specific tropism was not strictly correlated with the correspondent distribution of viral receptors in retina. These results provided important insights into the selection of appropriate vectors when specific retinal diseases are considered for gene therapy.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Curr Eye Res

DOI

ISSN

0271-3683

Publication Date

January 2008

Volume

33

Issue

1

Start / End Page

81 / 90

Location

England

Related Subject Headings

  • Tropism
  • Transduction, Genetic
  • Retina
  • Receptors, Vitronectin
  • Receptors, Virus
  • Rats, Sprague-Dawley
  • Rats
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Membrane Cofactor Protein
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, S.-H., Wu, J.-H., Wu, X.-B., Dong, X.-Y., Liu, X.-J., Li, C.-Y., & Qian-Huang. (2008). Distinctive gene transduction efficiencies of commonly used viral vectors in the retina. Curr Eye Res, 33(1), 81–90. https://doi.org/10.1080/02713680701799408
Zhang, Sheng-Hai, Ji-Hong Wu, Xiao-Bing Wu, Xiao-Yan Dong, Xin-Jian Liu, Chuan-Yuan Li, and Qian-Huang. “Distinctive gene transduction efficiencies of commonly used viral vectors in the retina.Curr Eye Res 33, no. 1 (January 2008): 81–90. https://doi.org/10.1080/02713680701799408.
Zhang S-H, Wu J-H, Wu X-B, Dong X-Y, Liu X-J, Li C-Y, et al. Distinctive gene transduction efficiencies of commonly used viral vectors in the retina. Curr Eye Res. 2008 Jan;33(1):81–90.
Zhang, Sheng-Hai, et al. “Distinctive gene transduction efficiencies of commonly used viral vectors in the retina.Curr Eye Res, vol. 33, no. 1, Jan. 2008, pp. 81–90. Pubmed, doi:10.1080/02713680701799408.
Zhang S-H, Wu J-H, Wu X-B, Dong X-Y, Liu X-J, Li C-Y, Qian-Huang. Distinctive gene transduction efficiencies of commonly used viral vectors in the retina. Curr Eye Res. 2008 Jan;33(1):81–90.
Journal cover image

Published In

Curr Eye Res

DOI

ISSN

0271-3683

Publication Date

January 2008

Volume

33

Issue

1

Start / End Page

81 / 90

Location

England

Related Subject Headings

  • Tropism
  • Transduction, Genetic
  • Retina
  • Receptors, Vitronectin
  • Receptors, Virus
  • Rats, Sprague-Dawley
  • Rats
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Membrane Cofactor Protein