Lupus autoantibodies recognize the product of an alternative open reading frame of SmB/B'.

Published

Journal Article

An unusual feature of the gene for the spliceosomal protein SmB/B' is the presence of an unusually long alternative open reading frame (aORF) which could encode 220 amino acids. We cloned and expressed this aORF protein and used immunological assays to determine its antigenicity in patients with systemic lupus. Sera from 10 of 22 (46%) anti-Sm positive lupus patients showed significant binding to the SmB' aORF protein by ELISA while neither the normal controls nor anti-Sm negative lupus patient controls showed significant reactivity. Antigenicity of the SmB' aORF protein was further localized to the C-terminus using a deletion construct. This is the first known example in which the product of an alternative open reading frame acts as an autoantigen in human disease. These results are consistent with the possibility that generation of anti-Sm autoantibodies in a subset of lupus patients is due to abnormal processing and expression of an aORF SmB/B' message, by an as yet unidentified mechanism.

Full Text

Duke Authors

Cited Authors

  • Kaufman, KM; Kirby, MY; McClain, MT; Harley, JB; James, JA

Published Date

  • August 2001

Published In

Volume / Issue

  • 285 / 5

Start / End Page

  • 1206 - 1212

PubMed ID

  • 11478783

Pubmed Central ID

  • 11478783

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.2001.5302

Language

  • eng