Skip to main content
Journal cover image

Lupus autoantibodies recognize the product of an alternative open reading frame of SmB/B'.

Publication ,  Journal Article
Kaufman, KM; Kirby, MY; McClain, MT; Harley, JB; James, JA
Published in: Biochem Biophys Res Commun
August 3, 2001

An unusual feature of the gene for the spliceosomal protein SmB/B' is the presence of an unusually long alternative open reading frame (aORF) which could encode 220 amino acids. We cloned and expressed this aORF protein and used immunological assays to determine its antigenicity in patients with systemic lupus. Sera from 10 of 22 (46%) anti-Sm positive lupus patients showed significant binding to the SmB' aORF protein by ELISA while neither the normal controls nor anti-Sm negative lupus patient controls showed significant reactivity. Antigenicity of the SmB' aORF protein was further localized to the C-terminus using a deletion construct. This is the first known example in which the product of an alternative open reading frame acts as an autoantigen in human disease. These results are consistent with the possibility that generation of anti-Sm autoantibodies in a subset of lupus patients is due to abnormal processing and expression of an aORF SmB/B' message, by an as yet unidentified mechanism.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 3, 2001

Volume

285

Issue

5

Start / End Page

1206 / 1212

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Serologic Tests
  • Ribonucleoproteins, Small Nuclear
  • Recombinant Fusion Proteins
  • Open Reading Frames
  • Molecular Sequence Data
  • Lupus Erythematosus, Systemic
  • Humans
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kaufman, K. M., Kirby, M. Y., McClain, M. T., Harley, J. B., & James, J. A. (2001). Lupus autoantibodies recognize the product of an alternative open reading frame of SmB/B'. Biochem Biophys Res Commun, 285(5), 1206–1212. https://doi.org/10.1006/bbrc.2001.5302
Kaufman, K. M., M. Y. Kirby, M. T. McClain, J. B. Harley, and J. A. James. “Lupus autoantibodies recognize the product of an alternative open reading frame of SmB/B'.Biochem Biophys Res Commun 285, no. 5 (August 3, 2001): 1206–12. https://doi.org/10.1006/bbrc.2001.5302.
Kaufman KM, Kirby MY, McClain MT, Harley JB, James JA. Lupus autoantibodies recognize the product of an alternative open reading frame of SmB/B'. Biochem Biophys Res Commun. 2001 Aug 3;285(5):1206–12.
Kaufman, K. M., et al. “Lupus autoantibodies recognize the product of an alternative open reading frame of SmB/B'.Biochem Biophys Res Commun, vol. 285, no. 5, Aug. 2001, pp. 1206–12. Pubmed, doi:10.1006/bbrc.2001.5302.
Kaufman KM, Kirby MY, McClain MT, Harley JB, James JA. Lupus autoantibodies recognize the product of an alternative open reading frame of SmB/B'. Biochem Biophys Res Commun. 2001 Aug 3;285(5):1206–1212.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 3, 2001

Volume

285

Issue

5

Start / End Page

1206 / 1212

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Serologic Tests
  • Ribonucleoproteins, Small Nuclear
  • Recombinant Fusion Proteins
  • Open Reading Frames
  • Molecular Sequence Data
  • Lupus Erythematosus, Systemic
  • Humans
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay