Quantitative and qualitative preparations of bacterial outer membrane vesicles.

Published

Journal Article

Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains.

Full Text

Duke Authors

Cited Authors

  • Chutkan, H; Macdonald, I; Manning, A; Kuehn, MJ

Published Date

  • January 2013

Published In

Volume / Issue

  • 966 /

Start / End Page

  • 259 - 272

PubMed ID

  • 23299740

Pubmed Central ID

  • 23299740

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-245-2_16

Language

  • eng