Quantitative and qualitative preparations of bacterial outer membrane vesicles.

Journal Article (Journal Article)

Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains.

Full Text

Duke Authors

Cited Authors

  • Chutkan, H; Macdonald, I; Manning, A; Kuehn, MJ

Published Date

  • 2013

Published In

Volume / Issue

  • 966 /

Start / End Page

  • 259 - 272

PubMed ID

  • 23299740

Pubmed Central ID

  • PMC4317262

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-245-2_16


  • eng

Conference Location

  • United States