Quantitative and qualitative preparations of bacterial outer membrane vesicles.
Publication
, Journal Article
Chutkan, H; Macdonald, I; Manning, A; Kuehn, MJ
Published in: Methods Mol Biol
2013
Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2013
Volume
966
Start / End Page
259 / 272
Location
United States
Related Subject Headings
- Microscopy, Electron
- Gram-Negative Bacteria
- Electrophoresis, Polyacrylamide Gel
- Developmental Biology
- Bacterial Outer Membrane Proteins
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Chutkan, H., Macdonald, I., Manning, A., & Kuehn, M. J. (2013). Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods Mol Biol, 966, 259–272. https://doi.org/10.1007/978-1-62703-245-2_16
Chutkan, Halima, Ian Macdonald, Andrew Manning, and Meta J. Kuehn. “Quantitative and qualitative preparations of bacterial outer membrane vesicles.” Methods Mol Biol 966 (2013): 259–72. https://doi.org/10.1007/978-1-62703-245-2_16.
Chutkan H, Macdonald I, Manning A, Kuehn MJ. Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods Mol Biol. 2013;966:259–72.
Chutkan, Halima, et al. “Quantitative and qualitative preparations of bacterial outer membrane vesicles.” Methods Mol Biol, vol. 966, 2013, pp. 259–72. Pubmed, doi:10.1007/978-1-62703-245-2_16.
Chutkan H, Macdonald I, Manning A, Kuehn MJ. Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods Mol Biol. 2013;966:259–272.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2013
Volume
966
Start / End Page
259 / 272
Location
United States
Related Subject Headings
- Microscopy, Electron
- Gram-Negative Bacteria
- Electrophoresis, Polyacrylamide Gel
- Developmental Biology
- Bacterial Outer Membrane Proteins
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences