Long-term in vivo imaging of multiple organs at the single cell level.

Journal Article

Two-photon microscopy has enabled the study of individual cell behavior in live animals. Many organs and tissues cannot be studied, especially longitudinally, because they are located too deep, behind bony structures or too close to the lung and heart. Here we report a novel mouse model that allows long-term single cell imaging of many organs. A wide variety of live tissues were successfully engrafted in the pinna of the mouse ear. Many of these engrafted tissues maintained the normal tissue histology. Using the heart and thymus as models, we further demonstrated that the engrafted tissues functioned as would be expected. Combining two-photon microscopy with fluorescent tracers, we successfully visualized the engrafted tissues at the single cell level in live mice over several months. Four dimensional (three-dimensional (3D) plus time) information of individual cells was obtained from this imaging. This model makes long-term high resolution 4D imaging of multiple organs possible.

Full Text

Duke Authors

Cited Authors

  • Chen, BJ; Jiao, Y; Zhang, P; Sun, AY; Pitt, GS; Deoliveira, D; Drago, N; Ye, T; Liu, C; Chao, NJ

Published Date

  • January 2, 2013

Published In

Volume / Issue

  • 8 / 1

Start / End Page

  • e52087 -

PubMed ID

  • 23300962

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

International Standard Serial Number (ISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0052087

Language

  • eng