Skip to main content

Long-term in vivo imaging of multiple organs at the single cell level.

Publication ,  Journal Article
Chen, BJ; Jiao, Y; Zhang, P; Sun, AY; Pitt, GS; Deoliveira, D; Drago, N; Ye, T; Liu, C; Chao, NJ
Published in: PLoS One
2013

Two-photon microscopy has enabled the study of individual cell behavior in live animals. Many organs and tissues cannot be studied, especially longitudinally, because they are located too deep, behind bony structures or too close to the lung and heart. Here we report a novel mouse model that allows long-term single cell imaging of many organs. A wide variety of live tissues were successfully engrafted in the pinna of the mouse ear. Many of these engrafted tissues maintained the normal tissue histology. Using the heart and thymus as models, we further demonstrated that the engrafted tissues functioned as would be expected. Combining two-photon microscopy with fluorescent tracers, we successfully visualized the engrafted tissues at the single cell level in live mice over several months. Four dimensional (three-dimensional (3D) plus time) information of individual cells was obtained from this imaging. This model makes long-term high resolution 4D imaging of multiple organs possible.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

1

Start / End Page

e52087

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Spleen
  • Myocardium
  • Models, Animal
  • Microscopy, Fluorescence, Multiphoton
  • Mice, Nude
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chen, B. J., Jiao, Y., Zhang, P., Sun, A. Y., Pitt, G. S., Deoliveira, D., … Chao, N. J. (2013). Long-term in vivo imaging of multiple organs at the single cell level. PLoS One, 8(1), e52087. https://doi.org/10.1371/journal.pone.0052087
Chen, Benny J., Yiqun Jiao, Ping Zhang, Albert Y. Sun, Geoffrey S. Pitt, Divino Deoliveira, Nicholas Drago, Tong Ye, Chen Liu, and Nelson J. Chao. “Long-term in vivo imaging of multiple organs at the single cell level.PLoS One 8, no. 1 (2013): e52087. https://doi.org/10.1371/journal.pone.0052087.
Chen BJ, Jiao Y, Zhang P, Sun AY, Pitt GS, Deoliveira D, et al. Long-term in vivo imaging of multiple organs at the single cell level. PLoS One. 2013;8(1):e52087.
Chen, Benny J., et al. “Long-term in vivo imaging of multiple organs at the single cell level.PLoS One, vol. 8, no. 1, 2013, p. e52087. Pubmed, doi:10.1371/journal.pone.0052087.
Chen BJ, Jiao Y, Zhang P, Sun AY, Pitt GS, Deoliveira D, Drago N, Ye T, Liu C, Chao NJ. Long-term in vivo imaging of multiple organs at the single cell level. PLoS One. 2013;8(1):e52087.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

1

Start / End Page

e52087

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Spleen
  • Myocardium
  • Models, Animal
  • Microscopy, Fluorescence, Multiphoton
  • Mice, Nude
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
  • Mice