Molecular genetic and immunohistochemical analysis of the tumor suppressor genes Rb and p53 in palmar and aggressive fibromatosis.

Published

Journal Article

This pilot project analyzed the tumor suppressor genes p53 and Rb in 13 cases of aggressive fibromatoses and six cases of palmar fibromatoses (Dupuytren contracture). Immunohistochemistry, reverse transcription polymerase chain reaction, polymerase chain reaction followed by single-strand confirmation polymorphism analysis, and Southern blot to detect gene rearrangements were used. No abnormalities were detected in p53. The aggressive fibromatoses demonstrated a lack of Rb immunohistochemical staining and decreased mRNA for Rb. No structural mutation in the coding sequence of the Rb gene was detected. The decreased level of Rb gene expression, despite a normal coding sequence, may indicate increased proliferation and may suggest potential treatment schemes.

Full Text

Duke Authors

Cited Authors

  • Muller, E; Castagnaro, M; Yandel, DW; Wolfe, HJ; Alman, BA

Published Date

  • September 1996

Published In

Volume / Issue

  • 5 / 3

Start / End Page

  • 194 - 200

PubMed ID

  • 8866233

Pubmed Central ID

  • 8866233

Electronic International Standard Serial Number (EISSN)

  • 1533-4066

International Standard Serial Number (ISSN)

  • 1052-9551

Digital Object Identifier (DOI)

  • 10.1097/00019606-199609000-00008

Language

  • eng